[Effects of total flavones of Elsholtzia splendens in isolated ischemia/reperfusion rat hearts].

Abstract

OBJECTIVE:

To investigate the influence of total flavonoids of Elsholtzia splendens (TFES) on isolated ischemia/reperfusion rat hearts and its underlying mechanisms.

METHODS:

Hearts isolated from male SD rats were perfused on the Langendorff apparatus and subjected to global ischemia for 30 min followed by 120 min of reperfusion. The cardiac infarct size was measured by TTC staining. Hemodynamic parameters and the level of lactate dehydrogenase (LDH) in the coronary effluent were measured. Absorbance at 520 nm was determined in isolated cardiac mitochondria exposed to 200 micromol/L CaCl2 to detect the opening of the mitochondrial permeability transition pore.

RESULTS:

Pretreatment with TFES (1, 10, 100 microg/ml) for 5 min decreased infarct size and LDH release and improved the recovery of the left ventricular developed pressure. In mitochondria, the decrease of absorbance at 520 nm evoked by CaCl2 was greatly inhibited by TFES.

CONCLUSION:

TFES prevents myocardial ischemia/reperfusion injury, and this cardioprotective effect is probably via inhibiting mitochondrial permeability transition pore opening.

• Authors: Liang Zhao, Zhi Li, Ling-Bo Qian, Zhi-Guo Ye, Guo-Lin Zhang, Qiang Xia, Hui-Ping Wang
• Journal: Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology (Zhongguo Ying Yong Sheng Li Xue Za Zhi) Vol. 30 Issue 2 Pg. 161-5 (Mar 2014) ISSN: 1000-6834 [Print] China
• PMID: 25016872 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cardiotonic Agents
• Flavones
• Mitochondrial Membrane Transport Proteins
• Mitochondrial Permeability Transition Pore

Topics

• Animals
• Cardiotonic Agents (pharmacology)
• Disease Models, Animal
• Flavones (pharmacology)
• In Vitro Techniques
• Lamiaceae (chemistry)
• Male
• Mitochondria, Heart (drug effects)
• Mitochondrial Membrane Transport Proteins (drug effects)
• Mitochondrial Permeability Transition Pore
• Myocardial Reperfusion
• Myocardial Reperfusion Injury (prevention & control)
• Rats
• Rats, Sprague-Dawley