Anti-apoptotic potency of TNFR:Fc gene in ischemia/ reperfusion-induced myocardial cell injury.

Abstract

The aim of the study was to investigate the anti-apoptotic potency of TNFR:Fc gene in ischemia/reperfusion-induced myocardial cell injury and hypoxia/reoxygenation-induced H9c2 rat cardiomyocytes injury. Rats were randomly divided into the following groups (n=8): (1) sham operation group; (2) ischemia-reperfusion (I/R) rats treated with rAAV-EGFP; (3) I/R rats treated with rAAV-TNFR:Fc group. rAAV-EGFP or rAAV-TNFR:Fc was injected intra-myocardial at four sites on the anterior and posterior walls of left ventricle immediately after the construction of I/R-induced AMI model in rats. The effects of TNFR:Fc on apoptosis and cardiacfunction were observed after 72 h of coronary reperfusion. In the in vitro study, apoptosis was analyzed in H9c2 rat cardiomyocytes treated either with nomoxia alone, or hypoxia/reoxygenation in the presence of rAAV-GFP or rAAV-TNFR:Fc. We found that (1) TNFR:Fc gene improved cardiac function (EF, LVESP, LVEDP and dp/dt max) post I/R-induced AMI; (2) TNFR:Fc gene inhibited I/R-induced apoptosis and attenuated the level of TNF-α in serum and cardiac tissue; (3) TNFR:Fc gene prevented apoptosis in hypoxia/reoxygenation-induced H9c2 rat cardiomyocytes associated with inhibition of caspase-3 activation and normalization of ratio of the Bcl-2/Bax. We concluded that TNFR:Fc gene transfection has anti-apoptotic potency in ischemia/reperfusion-induced myocardial cell injury.

• Authors: Jun Guo, Dong Zheng, Hai-Rui Li, Ai-Dong Zhang, Zi-Cheng Li
• Journal: Inflammation (Inflammation) Vol. 38 Issue 2 Pg. 664-71 (Apr 2015) ISSN: 1573-2576 [Electronic] United States
• PMID: 25015882 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Receptors, Fc
• Receptors, Tumor Necrosis Factor

Topics

• Animals
• Apoptosis (drug effects, genetics)
• Male
• Myocardial Reperfusion Injury (genetics, pathology, prevention & control)
• Myocytes, Cardiac (drug effects, pathology)
• Rats
• Rats, Sprague-Dawley
• Receptors, Fc (administration & dosage, genetics)
• Receptors, Tumor Necrosis Factor (administration & dosage, genetics)