Effect of Mailuoning injection on 8-iso-prostaglandin F2 alpha and superoxide dismutase in rabbits with extremity ischemia-reperfusion injury.

Abstract

BACKGROUND:

To date, there are no effective treatments for extremity ischemia-reperfusion (IR) injury. The objective of the present study was to explore the protective effect of Mailuoning on IR injury by investigating the plasma levels of 8-iso-prostaglandin F2 alpha (8-iso-PGF2α) and the activity of superoxide dismutase (SOD) in rabbits.

MATERIALS AND METHODS:

The experimental models of posterior limb IR injury were established in thirty rabbits that were divided into three groups: the sham, IR, and IR + Mailuoning groups. At the end of ischemia, Mailuoning was injected intravenously into the rabbits in the IR + Mailuoning group, and normal saline solution was administered to the rabbits in the sham and IR groups. Venous blood samples were collected to measure the levels of 8-iso-PGF2α and the activity of SOD in the plasma at the following time points: at the onset of ischemia, the end of ischemia, and 2, 4, 8, 12, and 24 h after reperfusion. The skeletal muscles were harvested to examine the ultrastructure.

RESULTS:

The levels of 8-iso-PGF2α increased significantly and SOD activity decreased in the IR group at every time point after reperfusion (P <0.01 or P <0.05). In contrast, the levels of 8-iso-PGF2α and SOD activity were not significantly different after reperfusion in the IR + Mailuoning group (P >0.05) but were significantly different compared with the IR group (P <0.01). Using electron microscopy, the skeletal muscle injury was shown to be milder in the IR+ Mailuoning group after reperfusion compared with the IR group.

CONCLUSIONS:

The Mailuoning is capable of decreasing the excessive production of 8-iso-PGF2α and protecting SOD activity, thereby exhibiting a protective effect on extremity IR injury.

• Authors: Dai-Jun Wang, Hua Tian
• Journal: The Journal of surgical research (J Surg Res) Vol. 192 Issue 2 Pg. 464-70 (Dec 2014) ISSN: 1095-8673 [Electronic] United States
• PMID: 25012271 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Drugs, Chinese Herbal
• mailuoning
• 8-epi-prostaglandin F2alpha
• Dinoprost
• Superoxide Dismutase

Topics

• Animals
• Cytoprotection (drug effects)
• Dinoprost (analogs & derivatives, metabolism)
• Disease Models, Animal
• Drugs, Chinese Herbal (pharmacology)
• Enzyme Activation (drug effects)
• Extremities (blood supply)
• Injections, Intralesional
• Male
• Microscopy, Electron, Transmission
• Muscle, Skeletal (blood supply, drug effects, ultrastructure)
• Rabbits
• Reperfusion Injury (drug therapy, metabolism)
• Superoxide Dismutase (metabolism)