Human papillomavirus infection requires the TSG101 component of the ESCRT machinery.

Abstract

Infection with human papillomaviruses (HPV) requires the minor capsid component L2, which plays an essential role in directing appropriate endosomal trafficking. Previous studies have indicated an infection route involving multi-vesicular bodies (MVBs), and an essential element in their biogenesis is the ESCRT machinery. Here we show that the ESCRT component TSG101 is required for optimal infection with both HPV-16 and BPV-1, with loss of TSG101 resulting in a decrease in viral infection, whereas overexpressed TSG101 increases rates of infection. We find that L2 proteins from multiple PV types interact with TSG101 and show that this interaction contributes to an alteration in the subcellular distribution of L2. In addition, TSG101 can modulate the levels of L2 polyubiquitination. These results demonstrate that TSG101 plays an important part in infection with diverse PVs, and suggests that trafficking of HPV through the ESCRT machinery and MVBs is part of infectious virus entry.

• Authors: Justyna Broniarczyk, Martina Bergant, Anna Goździcka-Józefiak, Lawrence Banks
• Journal: Virology (Virology) Vol. 460-461 Pg. 83-90 (Jul 2014) ISSN: 1096-0341 [Electronic] United States
• PMID: 25010273 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Capsid Proteins
• DNA-Binding Proteins
• Endosomal Sorting Complexes Required for Transport
• L2 protein, Human papillomavirus type 16
• Oncogene Proteins, Viral
• Transcription Factors
• Tsg101 protein

Topics

• Capsid Proteins (genetics, metabolism)
• DNA-Binding Proteins (genetics, metabolism)
• Endosomal Sorting Complexes Required for Transport (genetics, metabolism)
• Human papillomavirus 16 (genetics, physiology)
• Humans
• Oncogene Proteins, Viral (genetics, metabolism)
• Papillomavirus Infections (genetics, metabolism, virology)
• Protein Binding
• Transcription Factors (genetics, metabolism)