Abstract | PURPOSE: EXPERIMENTAL DESIGN: In this study, we aimed to assess the impact of Brachyury expression in prostate tumorigenesis using a large series of human prostate samples comprising benign tissue, prostate intraepithelial neoplasia (PIN) lesions, localized tumor, and metastatic tissues. The results obtained were compared with what can be inferred from the Oncomine database. In addition, multiple in vitro models of prostate cancer were used to dissect the biologic role of Brachyury in prostate cancer progression. RESULTS: We found that Brachyury is significantly overexpressed in prostate cancer and metastatic tumors when compared with normal tissues, both at protein and at mRNA levels. Brachyury expression in the cytoplasm correlates with highly aggressive tumors, whereas the presence of Brachyury in the nucleus is correlated with tumor invasion. We found that Brachyury-positive cells present higher viability, proliferation, migration, and invasion rates than Brachyury-negative cells. Microarray analysis further showed that genes co-expressed with Brachyury are clustered in oncogenic-related pathways, namely cell motility, cell-cycle regulation, and cell metabolism. CONCLUSIONS:
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Authors | Filipe Pinto, Nelma Pértega-Gomes, Márcia S Pereira, José R Vizcaíno, Pedro Monteiro, Rui M Henrique, Fátima Baltazar, Raquel P Andrade, Rui M Reis |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 20
Issue 18
Pg. 4949-61
(Sep 15 2014)
ISSN: 1557-3265 [Electronic] United States |
PMID | 25009296
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | ©2014 American Association for Cancer Research. |
Chemical References |
- Biomarkers, Tumor
- Fetal Proteins
- T-Box Domain Proteins
- Brachyury protein
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Topics |
- Adenocarcinoma
(pathology)
- Aged
- Biomarkers, Tumor
(analysis)
- Blotting, Western
- Cell Line, Tumor
- Fetal Proteins
(analysis, biosynthesis)
- Humans
- Immunohistochemistry
- Male
- Microscopy, Fluorescence
- Middle Aged
- Prognosis
- Prostatic Neoplasms
(pathology)
- Reverse Transcriptase Polymerase Chain Reaction
- T-Box Domain Proteins
(analysis, biosynthesis)
- Transfection
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