Antinociceptive effect of intrathecal microencapsulated human pheochromocytoma cell in a rat model of bone cancer pain.

Abstract

Human pheochromocytoma cells, which are demonstrated to contain and release met-enkephalin and norepinephrine, may be a promising resource for cell therapy in cancer-induced intractable pain. Intrathecal injection of alginate-poly (l) lysine-alginate (APA) microencapsulated human pheochromocytoma cells leads to antinociceptive effect in a rat model of bone cancer pain, and this effect was blocked by opioid antagonist naloxone and alpha 2-adrenergic antagonist rauwolscine. Neurochemical changes of cerebrospinal fluid are in accordance with the analgesic responses. Taken together, these data support that human pheochromocytoma cell implant-induced antinociception was mediated by met-enkephalin and norepinephrine secreted from the cell implants and acting at spinal receptors. Spinal implantation of microencapsulated human pheochromocytoma cells may provide an alternative approach for the therapy of chronic intractable pain.

• Authors: Xiao Li, Guoqi Li, Shaoling Wu, Baiyu Zhang, Qing Wan, Ding Yu, Ruijun Zhou, Chao Ma
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 15 Issue 7 Pg. 12135-48 (Jul 08 2014) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 25007069 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Alginates
• Biocompatible Materials
• Enkephalins
• alginate-polylysine-alginate
• Polylysine
• Yohimbine
• Naloxone
• Norepinephrine

Topics

• Adrenal Gland Neoplasms (metabolism, pathology)
• Alginates (chemistry)
• Animals
• Biocompatible Materials
• Bone Neoplasms (complications)
• Cell Transplantation
• Enkephalins (cerebrospinal fluid)
• Female
• Humans
• Naloxone (pharmacology)
• Nociceptive Pain (etiology, therapy)
• Norepinephrine (cerebrospinal fluid)
• Pheochromocytoma (metabolism, pathology)
• Polylysine (analogs & derivatives, chemistry)
• Rats
• Rats, Sprague-Dawley
• Spinal Cord (drug effects, metabolism)
• Yohimbine (pharmacology)