T-type Ca2+ channel blocker, KYS05090 induces autophagy and apoptosis in A549 cells through inhibiting glucose uptake.

Abstract

It has been reported that [3-(1,1'-biphenyl-4-yl)-2-(1-methyl-5-dimethylamino-pentylamino)-3,4-dihydroquinazolin-4-yl]-N-benzylacetamide 2hydrochloride (KYS05090), a selective T-type Ca2+ channel blocker, reduces tumor volume and weight in the A549 xenograft model, but the molecular mechanism of cell death has not yet been elucidated. In this study, KYS05090 induced autophagy- and apoptosis-mediated cell death in human lung adenocarcinoma A549 cells. Although KYS05090 decreased intracellular Ca2+ levels, it was not directly related with KYS05090-induced cell death. In addition, KYS05090 generated intracellular reactive oxygen species (ROS) and reduced glucose uptake, and catalase and methyl pyruvate prevented KYS05090-induced cell death. These results indicate that KYS05090 can lead to autophagy and apoptosis in A549 cells through ROS generation by inhibiting glucose uptake. Our findings suggest that KYS05090 has potential chemotherapeutic value for the treatment of lung cancer.

• Authors: Hong-Kun Rim, Sehyeon Cho, Dong-Hyun Shin, Kyung-Sook Chung, Young-Wuk Cho, Jung-Hye Choi, Jae Yeol Lee, Kyung-Tae Lee
• Journal: Molecules (Basel, Switzerland) (Molecules) Vol. 19 Issue 7 Pg. 9864-75 (Jul 08 2014) ISSN: 1420-3049 [Electronic] Switzerland
• PMID: 25006791 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Calcium Channel Blockers
• Calcium Channels, T-Type
• KYS 05090
• Quinazolines
• Reactive Oxygen Species
• Glucose
• Calcium

Topics

• Apoptosis (drug effects)
• Autophagy (drug effects)
• Calcium (metabolism)
• Calcium Channel Blockers (chemistry, pharmacology)
• Calcium Channels, T-Type
• Cell Line, Tumor
• Glucose (metabolism)
• Humans
• Quinazolines (chemistry, pharmacology)
• Reactive Oxygen Species (metabolism)