Abstract |
Punicalagin, a bioactive ellagitannin isolated from pomegranate, has been reported to have anti-inflammatory property. In the present study, we analyzed the role of punicalagin against acute respiratory distress syndrome (ARDS) induced by lipopolysaccharide (LPS) in mice. Male BALB/c mice with ARDS, induced by intranasal instillation of LPS, were treated with punicalagin 1 h prior to LPS exposure. The effects of punicalagin on pro-inflammatory cytokines, myeloperoxidase activity, nuclear factor kappa B (NF-κB) activation, and the histopathological changes were evaluated. The results showed that punicalagin treatment attenuated LPS-induced lung edema, elevating TNF-α, IL-6, and IL-1β levels in the bronchoalveolar lavage fluid (BALF). Meanwhile, punicalagin significantly inhibited LPS-induced increases in the macrophage and neutrophil infiltration of lung tissues and myeloperoxidase activity. Furthermore, punicalagin inhibits Toll-like receptor 4 (TLR4) expression and NF-κB activation induced by LPS. In conclusion, this is the first study to demonstrate that punicalagin protects against LPS-induced ARDS in mice. The underlying mechanisms may include inhibition of TLR4-mediated NF-κB signaling pathways.
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Authors | Jingjing Peng, Dong Wei, Zengqiang Fu, Dong Li, Yong Tan, Tao Xu, Jinjun Zhou, Tao Zhang |
Journal | Inflammation
(Inflammation)
Vol. 38
Issue 2
Pg. 493-9
(Apr 2015)
ISSN: 1573-2576 [Electronic] United States |
PMID | 25005005
(Publication Type: Journal Article)
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Chemical References |
- Anti-Inflammatory Agents
- Hydrolyzable Tannins
- IL1B protein, mouse
- Interleukin-1beta
- Interleukin-6
- Lipopolysaccharides
- NF-kappa B
- Tlr4 protein, mouse
- Toll-Like Receptor 4
- Tumor Necrosis Factor-alpha
- punicalagin
- Peroxidase
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Topics |
- Acute Lung Injury
(drug therapy)
- Animals
- Anti-Inflammatory Agents
(therapeutic use)
- Bronchoalveolar Lavage Fluid
(chemistry, cytology)
- Enzyme Activation
(drug effects)
- Hydrolyzable Tannins
(therapeutic use)
- Inflammation
(drug therapy)
- Interleukin-1beta
(metabolism)
- Interleukin-6
(metabolism)
- Lipopolysaccharides
- Lung
(immunology, pathology)
- Macrophages
(immunology)
- Male
- Mice
- Mice, Inbred BALB C
- NF-kappa B
(metabolism)
- Neutrophil Infiltration
(drug effects, immunology)
- Neutrophils
(immunology)
- Peroxidase
(metabolism)
- Pulmonary Edema
(drug therapy)
- Random Allocation
- Respiratory Distress Syndrome
(drug therapy)
- Toll-Like Receptor 4
(biosynthesis)
- Tumor Necrosis Factor-alpha
(metabolism)
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