Delphinidin suppresses PMA-induced MMP-9 expression by blocking the NF-κB activation through MAPK signaling pathways in MCF-7 human breast carcinoma cells.

Abstract

Matrix metalloproteinase-9 (MMP-9) plays an important role in the invasion and metastasis of cancer cells. The synthesis and secretion of MMP-9 can be stimulated by a variety of stimuli, including cytokines and phorbol 12-myristate 13-acetate (PMA), during various pathological processes, such as tumor invasion, atherosclerosis, inflammation, and rheumatoid arthritis, whereas MMP-2 is usually expressed constitutively. Delphinidin, an anthocyanidin present in pigmented fruits and vegetables, possesses potent antioxidant, anti-inflammatory, and antiangiogenic properties. In this study, we investigated the antiproliferative and antiinvasive effects of delphinidin on PMA-induced MMP-9 expression in MCF-7 human breast carcinoma cells using zymography, western blotting, reverse transcription-polymerase chain reaction, and Matrigel invasion assay. Delphinidin significantly suppressed PMA-induced MMP-9 protein expression in MCF-7 human breast carcinoma cells, and it also inhibited the MMP-9 gene transcriptional activity by blocking the activation of NFkappaB (NF-κB) through MAPK signaling pathways. Moreover, the Matrigel invasion assay showed that delphinidin reduces PMA-induced cancer cell invasion. These results suggest that delphinidin is a potential antimetastatic agent that suppresses PMA-induced cancer cell invasion through the specific inhibition of NF-κB-dependent MMP-9 gene expression.

• Authors: Nam-Kyung Im, Won Jun Jang, Chul-Ho Jeong, Gil-Saeng Jeong
• Journal: Journal of medicinal food (J Med Food) Vol. 17 Issue 8 Pg. 855-61 (Aug 2014) ISSN: 1557-7600 [Electronic] United States
• PMID: 25000305 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anthocyanins
• NF-kappa B
• Matrix Metalloproteinase 9
• delphinidin
• Tetradecanoylphorbol Acetate

Topics

• Anthocyanins (pharmacology)
• Breast Neoplasms (drug therapy, enzymology, genetics, physiopathology)
• Cell Proliferation (drug effects)
• Female
• Humans
• MAP Kinase Signaling System (drug effects)
• MCF-7 Cells
• Matrix Metalloproteinase 9 (genetics, metabolism)
• NF-kappa B (genetics, metabolism)
• Tetradecanoylphorbol Acetate (pharmacology)