HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Genkwadaphnin induces reactive oxygen species (ROS)-mediated apoptosis of squamous cell carcinoma (SCC) cells.

Abstract
Genkwadaphnin is a daphnane diterpene ester molecule isolated from the flower buds of Daphne genkwa. In the present study, we investigated the apoptosis-inducing effect of genkwadaphnin in squamous cell carcinoma (SCC) cells. Apoptosis was triggered in SCC12 cells following genkwadaphnin treatment in a time- and concentration-dependent manner. Genkwadaphnin treatment increased phosphorylation of c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase (MAPK). Knockdown of JNK and p38 MAPK by recombinant adenovirus expressing microRNA (miR) resulted in significant inhibition of genkwadaphnin-induced apoptosis in SCC12 cells. Finally, pretreatment with the reactive oxygen species (ROS) scavenger N-acetylcysteine (NAC) markedly reduced SCC12 cell apoptosis, concomitant with significant inhibition of MAPK activation. These results indicate that genkwadaphnin has the potential to induce apoptosis in SCC cells, providing information on which to base further research with the aim of developing a cure for SCC.
AuthorsZheng Jun Li, Xue Mei Li, Yong Jun Piao, Dae-Kyoung Choi, Sue Jeong Kim, Jae Wha Kim, Kyung-Cheol Sohn, Chang Deok Kim, Jeung-Hoon Lee
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 450 Issue 2 Pg. 1115-9 (Jul 25 2014) ISSN: 1090-2104 [Electronic] United States
PMID24996181 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2014 Elsevier Inc. All rights reserved.
Chemical References
  • Antineoplastic Agents
  • Diterpenes
  • Reactive Oxygen Species
  • genkwadaphnin
  • JNK Mitogen-Activated Protein Kinases
  • p38 Mitogen-Activated Protein Kinases
Topics
  • Antineoplastic Agents (pharmacology)
  • Apoptosis (drug effects)
  • Carcinoma, Squamous Cell (metabolism, pathology)
  • Cell Line, Tumor
  • Diterpenes (pharmacology)
  • Enzyme Activation
  • Gene Knockdown Techniques
  • Humans
  • JNK Mitogen-Activated Protein Kinases (genetics, metabolism)
  • Phosphorylation
  • Reactive Oxygen Species (metabolism)
  • Skin Neoplasms (metabolism, pathology)
  • p38 Mitogen-Activated Protein Kinases (genetics, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: