Pertussis toxin inhibits 1-methyladenine-induced maturation in starfish oocytes.

Abstract

Starfish oocytes injected with pertussis toxin (3-6 micrograms/ml) or its catalytically active A-subunit (1 microgram/ml) did not undergo germinal vesicle breakdown in response to 1-methyladenine (1-10 microM). The pertussis block could be bypassed by transfer of cytoplasm that contained maturation-promoting factor (MPF). After insemination, pertussis-blocked, MPF-rescued oocytes underwent cortical vesicle exocytosis and cleavage. These results suggest the involvement of a pertussis sensitive G-protein in the pathway coupling 1-methyladenine action at the cell surface to the reinitiation of meiosis.

• Authors: F Shilling, K Chiba, M Hoshi, T Kishimoto, L A Jaffe
• Journal: Developmental biology (Dev Biol) Vol. 133 Issue 2 Pg. 605-8 (Jun 1989) ISSN: 0012-1606 [Print] United States
• PMID: 2499496 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Growth Substances
• Virulence Factors, Bordetella
• 1-methyladenine
• Pertussis Toxin
• Maturation-Promoting Factor
• GTP-Binding Proteins
• Adenine

Topics

• Adenine (analogs & derivatives, pharmacology)
• Animals
• Cytoplasm
• Exocytosis
• Female
• GTP-Binding Proteins (physiology)
• Growth Substances (pharmacology)
• Maturation-Promoting Factor
• Oocytes (drug effects, physiology)
• Pertussis Toxin
• Starfish
• Virulence Factors, Bordetella (pharmacology)