Abstract | OBJECTIVE: MATERIALS AND METHODS:
Neuroblastoma cells were cultured and exposed to safranal (0, 10, 15, 20, 50 μg/ml). Cell proliferation was examined using the 3-(4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay. Apoptotic cells, cell cycle distribution, and sub-G1 fraction were analyzed using flow cytometric analysis after propidium iodide staining. RESULTS:
Safranal inhibited the growth of malignant cells in a dose-and time-dependent manner. The IC (50) values against the neuroblastoma cell line were determined as 11.1 and 23.3 μg/ml after 24 and 48 h, respectively. Safranal induced a sub-G1 peak in the flow cytometry histogram of treated cells compared to control cells indicating that apoptotic cell death is involved in safranal toxicity. CONCLUSIONS: Our pre-clinical study demonstrated a neuroblastoma cell line to be highly sensitive to safranal-mediated growth inhibition and apoptotic cell death. Although the molecular mechanisms of safranal action are not yet clearly understood, it appears to have potential as a therapeutic agent.
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Authors | Saeed Samarghandian, Mohammad Ebrahim Shoshtari, Javad Sargolzaei, Hosna Hossinimoghadam, Jabbari Azad Farahzad |
Journal | Pharmacognosy magazine
(Pharmacogn Mag)
Vol. 10
Issue Suppl 2
Pg. S419-24
(Apr 2014)
ISSN: 0973-1296 [Print] India |
PMID | 24991121
(Publication Type: Journal Article)
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