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[Inhibitory effect of alantolactone on the proliferation of K562/ADR cells and its mechanism].

AbstractOBJECTIVE:
To explore the inhibitory effect of alantolactone on the proliferation of adriamycin-resistant human chronic myelogenous leukemia cell line K562/ADR cells and its mechanism.
METHODS:
K562/ADR cells were treated with various concentrations of alantolactone (0, 1, 2, 4, 6, 8, and 10 μmol/L) for different time points. Cell viability was analyzed with MTT assay. The effect of alantolactone on the apoptosis of K562/ADR cells was measured by flow cytometry. The expression of apoptosis-related proteins after treatment with alantolactone was analyzed using Western blot.
RESULTS:
Alantolactone could effectively inhibit the proliferation of K562/ADR cells in dose- and time- dependent manner, the IC50 value of alantolactone treatment of K562/ADR cells for 24 h was 4.7 μmol/L (P<0.05). Flow cytometric analysis displayed that the apoptotic rates were 1.35%, 16.91%, 29.61% and 46.26%, respectively, after treatment with alantolactone at 0, 2.5, 5 and 7.5 μmol/L. Meanwhile, the expression of Bcl-2 and BCR-ABL proteins were significantly decreased and that of Bax, cytochrome C, cleaved-caspase-9, cleaved-caspase-3 and cleaved-PARP increased by alantolactone treatment.
CONCLUSION:
Alantolactone had obvious inhibitory effect on the proliferation of K562/ADR cells through the caspase dependent mitochondrial(or intrinsic)apoptotic pathway.
AuthorsChunhui Yang, Hong Cai, Jiangzhou Yan, Jingbo Yang, Meiyan Sun, Xiuxiang Meng, Tonghui Ma
JournalZhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (Zhonghua Xue Ye Xue Za Zhi) Vol. 35 Issue 6 Pg. 515-8 (Jun 2014) ISSN: 0253-2727 [Print] China
PMID24985175 (Publication Type: English Abstract, Journal Article)
Chemical References
  • BAX protein, human
  • Lactones
  • Proto-Oncogene Proteins c-bcl-2
  • Sesquiterpenes, Eudesmane
  • bcl-2-Associated X Protein
  • Fusion Proteins, bcr-abl
  • CASP3 protein, human
  • CASP9 protein, human
  • Caspase 3
  • Caspase 9
  • alantolactone
Topics
  • Apoptosis
  • Caspase 3 (metabolism)
  • Caspase 9 (metabolism)
  • Cell Proliferation (drug effects)
  • Fusion Proteins, bcr-abl (metabolism)
  • Humans
  • K562 Cells
  • Lactones (pharmacology)
  • Proto-Oncogene Proteins c-bcl-2 (metabolism)
  • Sesquiterpenes, Eudesmane (pharmacology)
  • bcl-2-Associated X Protein (metabolism)

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