Evaluation of commercial polyclonal- and monoclonal-antibody-based immunohistochemical tests for 2 genotypes of Porcine circovirus type 2 and comparison with in-situ hybridization assays.

Abstract

The objective of the present study was to evaluate polyclonal- and monoclonal-antibody-based immunohistochemical (IHC) tests for the detection of 2 genotypes of Porcine circovirus type 2 (PCV2), a and b, in formalin-fixed, paraffin-embedded lymph-node tissue from pigs with experimental or natural postweaning multisystemic wasting syndrome and to compare the IHC results with those of in-situ hybridization (ISH) assays. The ISH assays proved more sensitive than the IHC tests for the detection of PCV2a and PCV2b. According to these findings, polyclonal-antibody-based IHC testing is the most practical routine diagnostic method for the detection of PCV2 regardless of genotype because IHC testing is less technically complex than ISH testing. However, ISH assays are useful to differentiate between PCV2a and PCV2b in surveillance programs for the monitoring of PCV2 in swine herds.

• Authors: Hwi Won Seo, Kiwon Han, Yeonsu Oh, Ikjae Kang, Changhoon Park, Hye Eun Joo, Sung-Hoon Kim, Bog-Hieu Lee, Chanhee Chae
• Journal: Canadian journal of veterinary research = Revue canadienne de recherche veterinaire (Can J Vet Res) Vol. 78 Issue 3 Pg. 233-6 (Jul 2014) ISSN: 1928-9022 [Electronic] Canada
• PMID: 24982556 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Viral
• Formaldehyde

Topics

• Animals
• Antibodies, Monoclonal
• Antibodies, Viral
• Circovirus (classification, genetics)
• Formaldehyde
• Genotype
• Immunohistochemistry (veterinary)
• In Situ Hybridization (veterinary)
• Paraffin Embedding
• Reproducibility of Results
• Sensitivity and Specificity
• Swine
• Swine Diseases (virology)
• Tissue Fixation