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Analytical ultracentrifugation: a powerful 'new' technology in drug discovery.

Abstract
Analytical ultracentrifugation (AUC) is a powerful means of characterizing the solution behavior of molecules. Sedimentation velocity analysis, the preferred AUC technique for characterizing complex systems, has higher resolution, broader applicable range and fewer solute/solvent limitations than gel-permeation chromatography. The technique is simple to perform and should become a mainstay for target identification, target validation, lead optimization, formulation in drug development and QA/QC. Recent studies have used AUC to characterize the binding stoichiometry and binding sites of an anti-tumor agent; of a hemoglobin-stabilizing protein, and of a fibril growth inhibitor, and to assess the causes of protein aggregation. The recent addition of fluorescence to the existing absorbance and interference detectors dramatically extends the flexibility of analytical ultracentrifugation.:
AuthorsTom Laue
JournalDrug discovery today. Technologies (Drug Discov Today Technol) Vol. 1 Issue 3 Pg. 309-15 (Dec 2004) ISSN: 1740-6749 [Print] England
PMID24981500 (Publication Type: Journal Article)
Copyright© 2004 Elsevier Ltd . All rights reserved.

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