Abstract |
Analytical ultracentrifugation (AUC) is a powerful means of characterizing the solution behavior of molecules. Sedimentation velocity analysis, the preferred AUC technique for characterizing complex systems, has higher resolution, broader applicable range and fewer solute/ solvent limitations than gel-permeation chromatography. The technique is simple to perform and should become a mainstay for target identification, target validation, lead optimization, formulation in drug development and QA/QC. Recent studies have used AUC to characterize the binding stoichiometry and binding sites of an anti- tumor agent; of a hemoglobin-stabilizing protein, and of a fibril growth inhibitor, and to assess the causes of protein aggregation. The recent addition of fluorescence to the existing absorbance and interference detectors dramatically extends the flexibility of analytical ultracentrifugation.:
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Authors | Tom Laue |
Journal | Drug discovery today. Technologies
(Drug Discov Today Technol)
Vol. 1
Issue 3
Pg. 309-15
(Dec 2004)
ISSN: 1740-6749 [Print] England |
PMID | 24981500
(Publication Type: Journal Article)
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Copyright | © 2004 Elsevier Ltd . All rights reserved. |