Abstract | BACKGROUND: Cell-based assays for neuromyelitis optica (NMO) diagnosis are the most sensitive and specific methods to detect anti- aquaporin 4 (AQP4) antibodies in serum, but some improvements in their quantitative and specificity capacities would be desirable. Thus the aim of the present work was to develop a sensitive quantitative method for detection of anti-AQP4 antibodies that allows clear diagnosis of NMO and distinction of false labeling produced by natalizumab treatment. METHODS: RESULTS: Microscopic observation and fluorescence quantification produced similar results in fresh and frozen samples. Serum samples from patients diagnosed with NMO were 100% positive for anti-AQP4 antibodies, while all the other sera were negative. Using serum from patients treated with natalizumab, a small and unspecific fluorescent signal was produced from all HEK cells, regardless of AQP4 expression. CONCLUSIONS: Our cell-based double-label fluorescence immunoassay protocol significantly increases the signal specificity and reduces false diagnosis of NMO patients, especially in those receiving natalizumab treatment. Frozen pretreated cells allow faster detection of anti-AQP4 antibodies.
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Authors | Ismael Sánchez Gomar, María Díaz Sánchez, Antonio José Uclés Sánchez, José Luis Casado Chocán, Reposo Ramírez-Lorca, Ana Serna, Javier Villadiego, Juan José Toledo-Aral, Miriam Echevarría |
Journal | BMC neurology
(BMC Neurol)
Vol. 14
Pg. 139
(Jul 01 2014)
ISSN: 1471-2377 [Electronic] England |
PMID | 24980919
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- AQP4 protein, human
- Antibodies, Monoclonal, Humanized
- Aquaporin 4
- Autoantibodies
- Autoantigens
- Natalizumab
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Topics |
- Adolescent
- Adult
- Aged
- Antibodies, Monoclonal, Humanized
(blood, therapeutic use)
- Aquaporin 4
(blood)
- Autoantibodies
(blood)
- Autoantigens
(immunology)
- Female
- Fluorescent Antibody Technique
- Humans
- Immunoassay
(methods)
- Male
- Middle Aged
- Multiple Sclerosis
(drug therapy)
- Natalizumab
- Neuromyelitis Optica
(blood, diagnosis, immunology)
- Young Adult
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