Retracted: Guggulsterone inhibits prostate cancer growth via inactivation of Akt regulated by ATP citrate lyase signaling.

Abstract

We have shown previously that Gugggulsterone (Gug) inhibits growth of cultured LNCaP and PC-3 human prostate cancer cells by causing apoptosis induction in association with reactive-oxygen species (ROS)-dependent activation of c-Jun N-terminal kinase (JNK). The present study builds upon the novel observations and now reveals a novel mechanism of Gug-anticancer activity that ATP citrate lyase (ACLY)-regulated Akt inactivation is involved in Gug-mediated inhibition of prostate cancer growth. Oral gavage of Gug significantly retarded the growth of PC-3 xenografts in athymic mice without causing weight loss and any other side effects. The Gug-induced apoptosis was associated with remarkably down-regulation of Akt and ACLY in both cancer cells and xenografts tumor tissue of Gug-treated group. Ectopic expression of constitutively active Akt conferred significant protection against Gug-mediated apoptotic cell death in both cancer cells. Moreover, the Gug-induced apoptosis, and Akt and ACLY inactivation in PC-3 and LNCaP cells was intensified by siRNA-based knockdown of ACLY protein level and by pharmacological inhibition of ACLY, or was protected by the ectopic expression of ACLY. In conclusion, the present study reveals a novel mechanism of Gug-anticancer activity that Gug-inhibited prostate cancer growth is regulated by ACLY/Akt signaling axis.

• Authors: Yajuan Gao, Yan Zeng, Jian Tian, Mohammad Shyful Islam, Guoqin Jiang, Dong Xiao
• Journal: Oncotarget (Oncotarget) (Jun 26 2014) ISSN: 1949-2553 [Electronic] United States
• PMID: 24980815 (Publication Type: Journal Article, Retracted Publication)

Chemical References

• Pregnenediones
• pregna-4,17-diene-3,16-dione

Topics

• Humans
• Male
• Pregnenediones
• Prostatic Neoplasms