Airway
fibrosis, which is a crucial pathological condition occurring in various types of pulmonary disorders, is characterized by accumulation and activation of fibroblast cells, deposition of extracellular matrix (ECM)
proteins, and increase of airway basement membrane.
Transforming growth factor beta 1 (TGF-β1) is the principal profibrogenic
cytokine that is responsible for fibrotic responses. In the present study, we aimed to investigate the antifibrotic effects of the natural polyphenolic compound,
sesamin, on TGF-β1-induced fibroblast proliferation and activation, epithelial-mesenchymal transition (EMT), and
ovalbumin (OVA)-induced airway
fibrosis in vivo. We found that
sesamin attenuated TGF-β1-induced proliferation of cultured lung fibroblasts.
Sesamin inhibited TGF-β1-stimulated expression of alpha smooth muscle actin (α-SMA), suggesting that
sesamin plays an inhibitory role in fibroblast activation.
Sesamin blocked upregulation of the mesenchymal markers (
fibronectin and
vimentin) and downregulation of the epithelial marker (
E-cadherin), indicating an inhibitory effect on TGF-β1-induced EMT in A549 cells. TGF-β1-induced Smad3 phosphorylation was also significantly reduced by
sesamin in both cultured fibroblast and A549 cells. In the airway
fibrosis induced by OVA in mice,
sesamin inhibited the accumulation of α-SMA-positive cells and expression of
collagen I in the airway. Histological studies revealed that
sesamin protected against subepithelial
fibrosis by reducing myofibroblast activation and
collagen accumulation in the ECM. OVA-induced thickening of basement membrane was significantly alleviated in animals receiving
sesamin treatments. These results suggest a therapeutic potential of
sesamin as an antifibrotic agent.