Abstract | BACKGROUND: METHODS: Sixty-one rats were subjected to CA. After 6 min of ventricular fibrillation advanced cardiac life support was started. After successful return of spontaneous circulation (ROSC, n=40), animals were randomized either to placebo group (n=14) or AMP group (800 mg/kg body weight, n=14). Animals were kept at an ambient temperature of 18°C for 12 h after ROSC and core body temperature was measured using a telemetry temperature probe. Neuronal damage was analyzed by counting Nissl-positive (i.e. viable) neurons and TUNEL-positive (i.e. apoptotic) cells in coronal brain sections 7 days after ROSC. Functional status evaluated on days 1, 3 and 7 after ROSC by a tape removal test. RESULTS: Time until core body temperature dropped to <34.0°C was 31 min [28; 45] in AMP-treated animals and 125 min [90; 180] in the control group (p=0.003). Survival until 7 days after ROSC was comparable in both groups. Also number of Nissl-positive cells ( AMP: 1 [1; 7] vs. placebo: 2 [1; 3] per 100 pixel; p=0.66) and TUNEL-positive cells ( AMP: 56 [44; 72] vs. placebo: 53 [41; 67] per 100 pixel; p=0.70) did not differ. Neither did AMP affect functional neurological outcome up to 7 days after ROSC. Mean arterial pressure 20 min after ROSC was 49 [45; 55] mmHg in the AMP group in comparison to 91 [83; 95] mmHg in the control group (p<0.001). CONCLUSION: Although application of AMP reduced the time to reach a core body temperature of <34°C neither survival was improved nor neuronal damage attenuated. Reason for this is probably induction of marked hypotension as an adverse reaction to AMP treatment.
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Authors | Jürgen Knapp, Andreas Schneider, Corinna Nees, Thomas Bruckner, Bernd W Böttiger, Erik Popp |
Journal | Resuscitation
(Resuscitation)
Vol. 85
Issue 9
Pg. 1291-7
(Sep 2014)
ISSN: 1873-1570 [Electronic] Ireland |
PMID | 24978110
(Publication Type: Journal Article)
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Copyright | Copyright © 2014 Elsevier Ireland Ltd. All rights reserved. |
Chemical References |
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Topics |
- Adenosine Monophosphate
(therapeutic use)
- Animals
- Cardiopulmonary Resuscitation
(adverse effects)
- Heart Arrest
(therapy)
- Hypothermia, Induced
(adverse effects)
- Male
- Neurons
(pathology)
- Rats
- Rats, Wistar
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