Redox-responsive biodegradable PEGylated nanographene oxide for efficiently chemo-photothermal therapy: a comparative study with non-biodegradable PEGylated nanographene oxide.

Abstract

Nanographene oxide (NGO) with a non-sheddable poly(ethylene glycol) (PEG) coating has been used for chemo-photothermal therapy. However, the drug release of PEGylated NGO (NGO-PEG) with an amine bond is adversely affected by the diffusion barrier effect of PEG shells. Here, we developed a simple new method for the preparation of biodegradable PEGylated NGO conjugates (NGO-SS-PEG) with cleavable disulfide bonds for rapid drug release and more efficiently chemo-photothermal therapy. The glutathione (GSH)-induced and photothermal-mediated intracellular release of doxorubicin (DOX) from NGO-SS-PEG was studied in A549 cells using confocal laser scanning microscopy and flow cytometry analysis. In vivo cytotoxicity experiments were performed on chemo-photothermal therapy. Furthermore, we presented a comparative study of intracellular drug release and biological efficacy between NGO-SS-PEG/DOX and NGO-PEG/DOX. The results demonstrated that the rapid drug release from the NGO-SS-PEG conjugates with sheddable PEG was triggered upon the stimulus of high GSH levels inside A549 cells. Interesting, the DOX release mediated by the photothermal effect from the NGO-SS-PEG conjugates was found to be more obvious than that for NGO-PEG. Additionally, NGO-SS-PEG showed a higher efficacy than NGO-PEG for anti-tumor therapy compared with NGO-PEG. Thus, NGO-SS-PEG can improve therapeutic efficacy and is an attractive drug nanocarrier.

• Authors: Honglian Xiong, Zhouyi Guo, Wen Zhang, Huiqing Zhong, Songhao Liu, Yanhong Ji
• Journal: Journal of photochemistry and photobiology. B, Biology (J Photochem Photobiol B) Vol. 138 Pg. 191-201 (Sep 05 2014) ISSN: 1873-2682 [Electronic] Switzerland
• PMID: 24976623 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Coated Materials, Biocompatible
• Disulfides
• Drug Carriers
• Oxides
• Polyethylene Glycols
• Graphite
• Doxorubicin
• Glutathione

Topics

• Cell Line, Tumor
• Cell Survival (drug effects, radiation effects)
• Coated Materials, Biocompatible (chemistry)
• Disulfides (chemistry)
• Doxorubicin (administration & dosage, chemistry, pharmacology)
• Drug Carriers (chemistry)
• Glutathione (metabolism)
• Graphite (chemistry)
• Humans
• Lasers
• Nanoparticles (chemistry)
• Neoplasms (drug therapy, radiotherapy)
• Oxidation-Reduction
• Oxides (chemistry)
• Phototherapy
• Polyethylene Glycols (chemistry)
• Temperature