Collagenase-3 expression in periapical lesions: an immunohistochemical study.

Abstract

Collagenase-3 (matrix metalloproteinase-13) is a metalloproteinase (MMP) that is associated with bone lesions and exhibits variable expression patterns in odontogenic cysts; it may play a role in regulating focal proliferation and maturation of jaw cyst epithelium. We studied the localization, staining intensity and distribution of collagenase-3 in 13 periapical granulomas with epithelium, 16 periapical granulomas without epithelium and 10 radicular cysts using archived formalin fixed, paraffin embedded tissues. A monoclonal antibody against human collagenase-3 was used to evaluate its expression. Immunohistochemical staining intensities of collagenase-3 in all periapical lesions were (-), 4 (10%); (+), 1 (3%); (++), 22 (56%) and (+++), 12 (31%); differences were not statistically significant. Immunohistochemical distribution of collagenase-3 in epithelial cells was (-), 17 (44%); (+), 17 (44%); (++), 5 (13%); in fibroblasts it was (-), 8 (20%); (+), 23 (59%); (++), 8 (21%); in plasma cells it was (-), 7 (18%); (+), 22 (56%); (++), 10 (26%); in macrophages it was (-), 7 (18%); (+), and 15 (38%); and (++), 17 (44%). Statistically significant differences were found in epithelial cells (p = 0.00) and fibroblasts (p = 0.02), whereas differences were not statistically significant for plasma cells and macrophages. Collagenase-3 may play a role in the conversion of a periapical granuloma with epithelium to radicular cyst. MMP's influence not only epithelial rest cell migration, but also invasion of various stromal cells into granulomatous tissue.

• Authors: G Bhalla, M S Astekar, G Ramesh, P Kaur, G V Sowmya
• Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission (Biotech Histochem) Vol. 89 Issue 6 Pg. 457-63 (Aug 2014) ISSN: 1473-7760 [Electronic] England
• PMID: 24974940 (Publication Type: Journal Article)

Chemical References

• MMP13 protein, human
• Matrix Metalloproteinase 13

Topics

• Cell Movement (physiology)
• Epithelium (enzymology, pathology)
• Humans
• Immunohistochemistry (methods)
• Macrophages (metabolism)
• Matrix Metalloproteinase 13 (metabolism)
• Periapical Granuloma (enzymology, pathology)
• Radicular Cyst (enzymology, pathology)