Anti-hepatoma effect of safrole from Cinnamomum longepaniculatum leaf essential oil in vitro.

Abstract

The aim of this study was to study the anti-hepatoma effect of safrole and elucidate its molecular mechanism, the human hepatoma BEL-7402 cells were incubated with various concentrations (40, 80, 160, 320 and 640 μg/ml) of safrole and the cell proliferation and apoptosis were evaluated. The results showed that both the cell proliferation determined by 3-(4,5-dimethyl-thiazolyl-2)-2,5-diphenyl tetrazolium brominde (MTT) assay and cell colony determined by soft agar assay were significantly suppressed by safrole in a dose-time-dependent manner. Characteristic morphological and biochemical changes associated with apoptosis, including cells shrinkage, deformation and vacuolization of mitochondria, nuclear chromatin condensation and fragmentation, formation of apoptotic bodies were observed when treated with safrole for 24 h and 48 h. Cell cycle changes evaluated by flow cytometry analysis showed that the safrole could induce accumulation of cells arrested at G1 and S phases of the cell cycle. These results demonstrated that safrole is potent anti-hepatoma agent and the underlying mechanism may be attributed to suppress tumor cell growth by inducing cell apoptosis.

• Authors: Xu Song, Zhongqiong Yin, Kuichuan Ye, Qin Wei, Renrong Jia, Lijun Zhou, Yonghua Du, Jiao Xu, Xiaoxia Liang, Changliang He, Gang Shu, Lizi Yin, Cheng Lv
• Journal: International journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 7 Issue 5 Pg. 2265-72 ( 2014) ISSN: 1936-2625 [Electronic] United States
• PMID: 24966935 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Oils, Volatile
• Plant Oils
• Safrole

Topics

• Antineoplastic Agents, Phytogenic (isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Carcinoma, Hepatocellular (pathology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cinnamomum (chemistry)
• Dose-Response Relationship, Drug
• G1 Phase Cell Cycle Checkpoints (drug effects)
• Humans
• Liver Neoplasms (pathology)
• Mitochondria (drug effects, pathology)
• Oils, Volatile (chemistry)
• Phytotherapy
• Plant Leaves
• Plant Oils (chemistry)
• Plants, Medicinal
• S Phase Cell Cycle Checkpoints (drug effects)
• Safrole (isolation & purification, pharmacology)
• Time Factors