Investigation of genotoxicity in acromegaly from peripheral blood lymphocyte cultures using a micronucleus assay.

Abstract

CONTEXT:

Although patients with acromegaly may have an increased risk of developing several types of cancers, the degree of risk for malignancy in these patients is unresolved.

OBJECTIVE:

The present study aimed to investigate the potential genotoxic effects of acromegaly on the cell cycle in peripheral blood lymphocyte cultures.

DESIGN:

This was a single center, crossover, case-control study conducted on the acromegalic patients in Turkey.

SETTING:

The study was conducted in the outpatient clinic of a university hospital.

PATIENTS:

Seventy-one consecutively screened acromegalic patients and 56 controls participated in the study.

INTERVENTION:

Patients were included, regardless of the disease activity status and their treatment duration before the study.

MAIN OUTCOME MEASURES:

The primary end point was the frequency of micronucleus (MN) in the peripheral blood lymphocyte cultures, and the secondary end point was its clinical correlations.

RESULTS:

The MN level was 3.82 ± 1.49 in the control group and 18.00 ± 6.13 in the acromegalic group (P < .01), whereas the nuclear division index (NDI) was 1.79 ± 0.12 in the control group and 1.68 ± 0.07 in the acromegalic group (P < .01). Neither MN nor NDI was correlated with age, GH, IGF-I, initial GH, initial IGF-I, duration of the remission period, and initial tumor size. Only the MN level was positively correlated with the duration of disease (r = 0.323, P = .014).

CONCLUSION:

Our results indicated that acromegalic patients had genotoxic damage at a substantial level, and there was a positive correlation between the duration of disease and genotoxicity level.

• Authors: Oguz Kaan Unal, Nilufer Cinkilic, Ozen Oz Gul, Soner Cander, Ozgur Vatan, Canan Ersoy, Dilek Yilmaz, Ercan Tuncel
• Journal: The Journal of clinical endocrinology and metabolism (J Clin Endocrinol Metab) Vol. 99 Issue 10 Pg. E2060-6 (Oct 2014) ISSN: 1945-7197 [Electronic] United States
• PMID: 24963647 (Publication Type: Journal Article)

Chemical References

• Human Growth Hormone
• Insulin-Like Growth Factor I

Topics

• Acromegaly (epidemiology, genetics)
• Adult
• Case-Control Studies
• Cells, Cultured
• Cross-Over Studies
• Female
• Human Growth Hormone (genetics)
• Humans
• Insulin-Like Growth Factor I (genetics)
• Lymphocytes (cytology, physiology)
• Male
• Micronucleus Tests (methods)
• Middle Aged
• Mutagenicity Tests
• Neoplasms (epidemiology, genetics)
• Risk Factors
• Turkey (epidemiology)