[Anti-atherosclerosis role of N-oleoylethanolamine in CB2].

Abstract

To observe a PPAR-alpha agonist effect of N-oleoylethanolamine (OEA) on CB2 (cannabinoid receptor 2), an anti-inflammatory receptor in vascular endothelial cell, healthy HUVECs and TNF-alpha induced HUVECs were used to establish a human vascular endothelial cell inflammatory model. Different doses of OEA (10, 50 and 100 micromol x L(-1)) had been given to HUVECs, cultured at 37 degrees C for 7 h and then collected the total protein and total mRNA. CB2 protein expression was detected by Western blotting and CB2 mRNA expression was assayed by real-time PCR. As the results shown, OEA (10 and 50 micromol x L(-1)) could induce the CB2 protein and mRNA expression, but not 100 micromol x L(-1). To detect if anti-inflammation effect of OEA is partly through CB2, CB2 inhibitor AM630 was used to inhibit HUVEC CB2 expression, then the VCAM-1 expression induced by TNF-alpha was detected, or THP-1 adhere to TNF-alpha induced HUVECs was examined. OEA (50 micromol x L(-1)) could inhibit TNF-alpha induced VCAM-1 expression and THP-1 adhere to HUVECs, these effects could be partly inhibited by a CB2 inhibitor AM630. The anti-inflammation effect of OEA is induced by PPAR-alpha and CB2, suggesting that CB2 signaling could be a target for anti-atherosclerosis, OEA have wide effect in anti-inflammation, it may have better therapeutic potential in anti-inflammation in HUVECs, thus achieving anti-atherosclerosis effect.

• Authors: Ya-Ting Gai, Qiang Shu, Cai-Xia Chen, You-Lin Lai, Wen-Jun Li, Lu Peng, Li-Min Lin, Xin Jin
• Journal: Yao xue xue bao = Acta pharmaceutica Sinica (Yao Xue Xue Bao) Vol. 49 Issue 3 Pg. 316-21 (Mar 2014) ISSN: 0513-4870 [Print] China
• PMID: 24961101 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Endocannabinoids
• Ethanolamines
• Indoles
• Oleic Acids
• PPAR alpha
• RNA, Messenger
• Receptor, Cannabinoid, CB2
• Tumor Necrosis Factor-alpha
• Vascular Cell Adhesion Molecule-1
• MK-886
• N-oleoylethanolamine
• iodopravadoline

Topics

• Anti-Inflammatory Agents (pharmacology)
• Atherosclerosis (pathology)
• Cell Adhesion (drug effects)
• Cells, Cultured
• Endocannabinoids (pharmacology)
• Endothelial Cells (cytology, metabolism)
• Ethanolamines (pharmacology)
• Humans
• Indoles (pharmacology)
• Monocytes (drug effects)
• Oleic Acids (pharmacology)
• PPAR alpha (antagonists & inhibitors)
• RNA, Messenger (metabolism)
• Receptor, Cannabinoid, CB2 (antagonists & inhibitors, genetics, metabolism)
• Tumor Necrosis Factor-alpha (pharmacology)
• Vascular Cell Adhesion Molecule-1 (metabolism)