HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Structure and negative transcriptional regulation by glucocorticoids of the acute-phase rat alpha 1-inhibitor III gene.

Abstract
DNA clones representing the negative acute-phase gene coding for the plasma proteinase inhibitor alpha 1-inhibitor III were isolated from a rat genomic library. Structural analysis established the existence of at least four different members of the alpha 1-inhibitor III gene family. Partial DNA sequence analysis of the 5'-terminal regions was performed for the alpha 1-inhibitor III gene and the related alpha 1-inhibitor IV gene. The transcription start site of the alpha 1-inhibitor III gene was located by S1 mapping and primer extension. No stable alpha 1-inhibitor IV mRNA was detected in rat liver. In an experimentally induced acute-phase reaction, the transcription rate of the alpha 1-inhibitor III gene was reduced 12.7-fold at 6 h after stimulation. Four hours after injection of a high dose of dexamethasone into rats, the transcription rate of this gene was reduced 9-fold. Thus, glucocorticoids alone are capable of causing a strong transient down-regulation of the transcription of this gene in rats, independent of other inflammatory mediators. An inverted consensus glucocorticoid responsive element (5'GGACACAATAT3') shared with the glucocorticoid-regulated alpha 1-fetoprotein, alpha 2u-globulin, and alpha 1-acid glycoprotein genes was detected by computer-assisted sequence analysis in the promoter proximal 5'-flanking region of the alpha 1-inhibitor III gene.
AuthorsW Northemann, B R Shiels, T A Braciak, G H Fey
JournalBiochemistry (Biochemistry) Vol. 28 Issue 1 Pg. 84-95 (Jan 10 1989) ISSN: 0006-2960 [Print] United States
PMID2495816 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Acute-Phase Proteins
  • Protease Inhibitors
  • alpha 1-inhibitor 3
  • Dexamethasone
  • DNA
Topics
  • Acute-Phase Proteins
  • Acute-Phase Reaction (genetics)
  • Animals
  • Base Sequence
  • DNA (genetics)
  • Dexamethasone (pharmacology)
  • Male
  • Molecular Sequence Data
  • Multigene Family
  • Protease Inhibitors (genetics)
  • Rats
  • Rats, Inbred F344
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic (drug effects)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: