Abstract | BACKGROUND: Lathyrus species as legumes represent an alternative protein source for human and animal nutrition. Heavy consumption of these species can lead to lathyrism, caused by the non- protein amino acid β-N-oxalyl-l-α,β-diaminopropionic acid (β- ODAP). Currently, there is no well-defined level below which β- ODAP is considered non-toxic. In this work, the β- ODAP content was determined in L. sativus and L. cicera samples to assess their potential toxicity. Homoarginine is another non- protein amino acid found in Lathyrus spp. with interesting implications for human and animal nutrition. RESULTS: The level of β- ODAP found in these two species ranged from 0.79 to 5.05 mg g(-1). The homoarginine content of the samples ranged from 7.49 to 12.44 mg g(-1). CONCLUSION: This paper describes an accurate, fast and sensitive method of simultaneous detection and quantification of β- ODAP and homoarginine by capillary zone electrophoresis in L. cicera and L. sativus seeds. Moreover, several methods of extraction were compared to determine the highest performance.
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Authors | Mara Sacristán, Alejandro Varela, Mercedes M Pedrosa, Carmen Burbano, Carmen Cuadrado, M Estrella Legaz, Mercedes Muzquiz |
Journal | Journal of the science of food and agriculture
(J Sci Food Agric)
Vol. 95
Issue 7
Pg. 1414-20
(May 2015)
ISSN: 1097-0010 [Electronic] England |
PMID | 24954190
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 Society of Chemical Industry. |
Chemical References |
- Dietary Proteins
- beta-Alanine
- Homoarginine
- 2,3-diaminopropionic acid
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Topics |
- Animals
- Diet
- Dietary Proteins
- Electrophoresis, Capillary
(methods)
- Homoarginine
(adverse effects, analysis)
- Humans
- Lathyrism
(etiology)
- Lathyrus
(adverse effects, chemistry)
- Seeds
(chemistry)
- beta-Alanine
(adverse effects, analogs & derivatives, analysis)
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