Research on a novel poly (vinyl alcohol)/lysine/vanillin wound dressing: Biocompatibility, bioactivity and antimicrobial activity.

Abstract

Burn wound dressings have played significant roles in daily clinical practice. An "ideal" burn wound dressing is non-adhesion, absorbency and antimicrobial activity. However, such a dressing is currently not available. A novel composite hydrogel was based on poly (vinyl alcohol) (PVA) containing lysine (Lys) and vanillin (V) using freezing-thawing method. The properties of this hydrogel were characterized by environmental scanning electron microscope (ESEM), attenuated total reflectance fourier transform infrared spectroscopy (ATR-FTIR), tensile testing, differential scanning calorimetry (DSC) and water vapour transmission rate (WVTR). Then the antibacterial activity of PVA/Lys/V composite hydrogel was examined by bacteriolytic plate. In vivo experiment, a burn rat model was used to evaluate the histological analysis of this hydrogel. In results, the Schiff base formed in the three-phase system. It improved the tensile strength and crystallization of the PVA/Lys/V composite hydrogel. Meanwhile, this hydrogel showed excellent bactericidal activities to both gram-negative bacteria (E. coli) and gram-positive bacteria (S. aureus) due to the Schiff base. And the antibacterial activity toward gram-negative bacteria was better than another. On Day 7, 95-100% of the surface areas of PVA/Lys/V composite treated burns were covered with regenerating epidermis. And the new tissue and capillary vessel formed around the wounds after treatment. Therefore, it is suggested that treatment with PVA/Lys/V composite hydrogel will be effective also in patients with burns and other skin wounds.

• Authors: Gang Zhou, Ruhan A, Heng Ge, Lin Wang, Meili Liu, Binbin Wang, Haisheng Su, Ming Yan, Yuan Xi, Yubo Fan
• Journal: Burns : journal of the International Society for Burn Injuries (Burns) Vol. 40 Issue 8 Pg. 1668-78 (Dec 2014) ISSN: 1879-1409 [Electronic] Netherlands
• PMID: 24953436 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Ltd and ISBI. All rights reserved.

Chemical References

• Anti-Bacterial Agents
• Antioxidants
• Benzaldehydes
• Hydrogels
• Polyvinyl Alcohol
• vanillin
• Lysine

Topics

• Animals
• Anti-Bacterial Agents (pharmacology)
• Antioxidants (pharmacology)
• Bandages
• Benzaldehydes (pharmacology)
• Burns (therapy)
• Disease Models, Animal
• Escherichia coli (drug effects)
• Hydrogels (pharmacology)
• Lysine (pharmacology)
• Materials Testing
• Microbial Sensitivity Tests
• Microscopy, Electron, Scanning
• Polyvinyl Alcohol (pharmacology)
• Rats
• Staphylococcus aureus (drug effects)
• Tensile Strength
• Wound Healing (drug effects)