Mitochondrial protein cyclophilin-D-mediated programmed necrosis attributes to berberine-induced cytotoxicity in cultured prostate cancer cells.

Abstract

The prostate cancer is one of the leading causes of men's cancer mortality. The development of alternative chemotherapeutic strategies is urgent. Berberine has displayed significant anti-prostate cancer activities. The underlying mechanisms are not fully understood. In the current study, we found that berberine induced apoptosis and programmed necrosis in cultured prostate cancer cells (LNCaP and PC-82 lines), and necrosis weighted more than apoptosis in contributing berberine's cytotoxicity. We demonstrated that mitochondrial protein cyclophilin-D (Cyp-D) is required for berberine-induced programmed necrosis. Inhibition of Cyp-D by its inhibitors cyclosporin A (CSA) or sanglifehrin A (SFA), and by Cyp-D shRNA depletion alleviated berberine-induced prostate cancer cell necrosis (but not apoptosis). Our data found that in prostate cancer cells, berberine induced reactive oxygen species (ROS) production, which dictated P53 translocation to mitochondria, where it physically interacted with Cyp-D to open mitochondrial permeability transition pore (mPTP). The anti-oxidant N-acetylcysteine (NAC), the P53 inhibitor pifithrin-α (PFTα) as well as P53 siRNA knockdown suppressed berberine-induced P53 mitochondrial translocation and Cyp-D association, thus inhibiting mitochondrial membrane potential (MMP) decrease and prostate cancer cell necrosis. In summary, the results of the present study provide mechanistic evidence that both apoptosis and programmed necrosis attribute to berberine's cytotoxicity in prostate cancer cells.

• Authors: Long-yang Zhang, Yan-lin Wu, Xing-hua Gao, Feng Guo
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 450 Issue 1 Pg. 697-703 (Jul 18 2014) ISSN: 1090-2104 [Electronic] United States
• PMID: 24946211 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier Inc. All rights reserved.

Chemical References

• Mitochondrial Proteins
• Reactive Oxygen Species
• TP53 protein, human
• Tumor Suppressor Protein p53
• Berberine
• Cyclophilins
• PPID protein, human

Topics

• Berberine (pharmacology)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Cyclophilins (metabolism)
• Humans
• Male
• Mitochondrial Proteins (metabolism)
• Necrosis (chemically induced, metabolism, pathology)
• Prostatic Neoplasms (metabolism, pathology)
• Reactive Oxygen Species (metabolism)
• Tumor Suppressor Protein p53 (metabolism)