Abstract | BACKGROUND: STUDY DESIGN AND METHODS:
Hypoxia-induced NO bioavailability and methemoglobin formation were measured in vitro after nitrite administration to fresh (<1 week of storage) and aged (5-6 weeks of storage) human RBC units and in blood samples of hemodiluted rats subjected to hypoxic ventilation after transfusion with fresh or aged human RBCs. RESULTS: In vitro, NO and methemoglobin levels 10 minutes after nitrite administration were lower in the fresh RBC samples compared to the aged RBC samples (p = 0.026 and p = 0.022, respectively). In vivo, NO bioavailability was also significantly lower in the rats receiving fresh RBCs compared to the group receiving aged RBCs (p = 0.003). In line with NO bioavailability, methemoglobin levels were higher, albeit not significantly, in the group receiving aged RBCs compared to in the group receiving fresh RBCs (p = 0.154). The difference in methemoglobin formation after nitrite administration between fresh and aged RBCs was only present under deoxygenated conditions and not under oxygenated conditions. There were no differences in methemoglobin reductase activity between fresh and aged RBCs. CONCLUSIONS: Storage of RBCs leads to an increased rate of hypoxia-induced nitrite reduction to NO and this is associated with increased methemoglobin formation. The increased methemoglobin formation and consequent decrease in oxygen delivery capacity might contribute to the storage-related impairment of aged RBCs to oxygenate the microcirculation.
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Authors | Emre Almac, Rick Bezemer, Petra M Hilarius-Stokman, Peter Goedhart, Dirk de Korte, Arthur J Verhoeven, Can Ince |
Journal | Transfusion
(Transfusion)
Vol. 54
Issue 12
Pg. 3178-85
(Dec 2014)
ISSN: 1537-2995 [Electronic] United States |
PMID | 24942042
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2014 AABB. |
Chemical References |
- Nitric Oxide
- Methemoglobin
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Topics |
- Animals
- Biological Availability
- Blood Preservation
- Cell Hypoxia
- Erythrocytes
(cytology, metabolism)
- Humans
- Male
- Methemoglobin
(metabolism)
- Nitric Oxide
(metabolism)
- Rats
- Rats, Wistar
- Time Factors
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