Abstract | OBJECTIVE: METHODS: Immunofluorescence laser was used to confirm CASK and Id1 protein expression and localization in fibroblasts of the keloid and normal skin. RT-PCR and Western-blot were adopted to analysis the CASK and Id1 expression and differences between keloid and normal skin fibroblasts. The natural combination of CASK and Id1 protein of keloid fibroblasts was tested by immunoprecipitation. RESULTS: CASK and Id1 protein expression were both found in fibroblast cells of keloid and normal skin under normal circumstances. Most of CASK and Id1 were distributed in the cytoplasm and nucleus of fibroblasts. The results of RT-PCR showed that the expression of CASK mRNA in the keloid group was 0.658 +/- 0.024, which was lower than that in the normal control group (1.076 +/- 0.008, t = 11.159, P < 0.05). The expression of Id1 mRNA was 0.497 +/- 0.014, which was higher than that in the normal control group (0.307 +/- 0.017, t = 15.148, P < 0.05). The results of Western-blot showed that the expression level for CASK protein in the keloid group was 0.057 +/- 0.006, which was lower than that in the normal control group (0.168 +/- 0.012, t = 13.524, P < 0.05); the expression of Id1 protein was 0.812 +/- 0.035, which was higher than that in the normal control group (0.368 +/- 0.031, t = 16.356, P < 0.05). The results of immunoprecipitation showed that Id1 could be detected in the CASK precipitate, while CASK also could be detected in the Id1 precipitate. There was a natural binding of CASK and Id1 in keloid fibroblasts. CONCLUSION: CASK/Id1 signal pathway may be existed and involved in the proliferation of keloid fibroblasts, which is related with the occurrence of keloid.
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Authors | Liang Xiao, Zhibo Sun, Yong Ke, Zhihong Yu, Guangzhao He, Yuhan Ren |
Journal | Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery
(Zhonghua Zheng Xing Wai Ke Za Zhi)
Vol. 30
Issue 2
Pg. 105-11
(Mar 2014)
ISSN: 1009-4598 [Print] China |
PMID | 24941761
(Publication Type: Journal Article)
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Chemical References |
- Cyclin-Dependent Kinase Inhibitor Proteins
- Inhibitor of Differentiation Protein 1
- RNA, Messenger
|
Topics |
- Cell Proliferation
(genetics)
- Cyclin-Dependent Kinase Inhibitor Proteins
(genetics, metabolism)
- Fibroblasts
(metabolism)
- Humans
- Inhibitor of Differentiation Protein 1
(genetics, metabolism)
- Keloid
(metabolism, pathology)
- RNA, Messenger
(metabolism)
- Signal Transduction
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