Abstract | BACKGROUND: The present study was aimed to evaluate whether IgG, IgM and IgA antibodies levels detected against a novel Mycobacterium tuberculosis polyprotein 38 F-64 F (with 38 F being the abbreviation for 38kD-ESAT6-CFP10 and 64 F for Mtb8.4-MPT64-TB16.3-Mtb8) are suitable for diagnosing active tuberculosis, and for monitoring the efficacy of chemotherapy on TB patients. METHODS: In this study, a total of 371 active TB patients without treatment were selected and categorized into S+/C+group (n=143), S-/C+group (n=106) or S-/C- group (n=122). A series of serum samples were collected from 82 active TB patients who had undergone anti-TB chemotherapy for 0-6 months at one month interval. Humoral responses ( IgG, IgM and IgA) were determined for the novel Mycobacterium tuberculosis polyprotein using indirect ELISA methods in all of serum samples. RESULTS: For S+/C+, S-/C+and S-/C- active tuberculosis patients before anti-TB chemotherapy, the sensitivities of tests based on IgG were 65.7%, 46.2% and 52.5% respectively; the sensitivities based on IgM were 21.7%, 24.5% and 18.9%; and the sensitivities based on IgA were 25.2%, 17.9% and 23.8%. By combination of three isotypes, for all active tuberculosis patients, the test sensitivity increased to 70.4% with the specificity being 91.5%. After anti-TB chemotherapy, there were no significant differences between groups with different courses of anti-TB chemotherapy. CONCLUSIONS:
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Authors | Xiaoyan Feng, Xiqin Yang, Bingshui Xiu, Shuang Qie, Zhenhua Dai, Kun Chen, Ping Zhao, Li Zhang, Russell A Nicholson, Guohua Wang, Xiaoguo Song, Heqiu Zhang |
Journal | BMC infectious diseases
(BMC Infect Dis)
Vol. 14
Pg. 336
(Jun 17 2014)
ISSN: 1471-2334 [Electronic] England |
PMID | 24939009
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Bacterial
- Antitubercular Agents
- Bacterial Proteins
- Immunoglobulin A
- Immunoglobulin G
- Immunoglobulin M
- Polyproteins
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Topics |
- Adult
- Aged
- Antibodies, Bacterial
(blood, immunology)
- Antitubercular Agents
(therapeutic use)
- Bacterial Proteins
(immunology)
- Drug Monitoring
- Enzyme-Linked Immunosorbent Assay
- Female
- Humans
- Immunoglobulin A
(blood, immunology)
- Immunoglobulin G
(blood, immunology)
- Immunoglobulin M
(blood, immunology)
- Male
- Middle Aged
- Mycobacterium tuberculosis
(immunology, isolation & purification)
- Polyproteins
(immunology)
- Tuberculosis
(blood, diagnosis, drug therapy, immunology, microbiology)
- Young Adult
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