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IgG, IgM and IgA antibodies against the novel polyprotein in active tuberculosis.

AbstractBACKGROUND:
The present study was aimed to evaluate whether IgG, IgM and IgA antibodies levels detected against a novel Mycobacterium tuberculosis polyprotein 38 F-64 F (with 38 F being the abbreviation for 38kD-ESAT6-CFP10 and 64 F for Mtb8.4-MPT64-TB16.3-Mtb8) are suitable for diagnosing active tuberculosis, and for monitoring the efficacy of chemotherapy on TB patients.
METHODS:
In this study, a total of 371 active TB patients without treatment were selected and categorized into S+/C+group (n=143), S-/C+group (n=106) or S-/C- group (n=122). A series of serum samples were collected from 82 active TB patients who had undergone anti-TB chemotherapy for 0-6 months at one month interval. Humoral responses (IgG, IgM and IgA) were determined for the novel Mycobacterium tuberculosis polyprotein using indirect ELISA methods in all of serum samples.
RESULTS:
For S+/C+, S-/C+and S-/C- active tuberculosis patients before anti-TB chemotherapy, the sensitivities of tests based on IgG were 65.7%, 46.2% and 52.5% respectively; the sensitivities based on IgM were 21.7%, 24.5% and 18.9%; and the sensitivities based on IgA were 25.2%, 17.9% and 23.8%. By combination of three isotypes, for all active tuberculosis patients, the test sensitivity increased to 70.4% with the specificity being 91.5%. After anti-TB chemotherapy, there were no significant differences between groups with different courses of anti-TB chemotherapy.
CONCLUSIONS:
The novel Mycobacterium tuberculosis polyprotein 38 F-64 F represents potential antigen suitable for measuring IgG, IgM and IgA antibodies. However, the serodiagnostic test based on the 38 F-64 F polyprotein appears unsuitable for monitoring the efficacy of chemotherapy.
AuthorsXiaoyan Feng, Xiqin Yang, Bingshui Xiu, Shuang Qie, Zhenhua Dai, Kun Chen, Ping Zhao, Li Zhang, Russell A Nicholson, Guohua Wang, Xiaoguo Song, Heqiu Zhang
JournalBMC infectious diseases (BMC Infect Dis) Vol. 14 Pg. 336 (Jun 17 2014) ISSN: 1471-2334 [Electronic] England
PMID24939009 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Bacterial
  • Antitubercular Agents
  • Bacterial Proteins
  • Immunoglobulin A
  • Immunoglobulin G
  • Immunoglobulin M
  • Polyproteins
Topics
  • Adult
  • Aged
  • Antibodies, Bacterial (blood, immunology)
  • Antitubercular Agents (therapeutic use)
  • Bacterial Proteins (immunology)
  • Drug Monitoring
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Immunoglobulin A (blood, immunology)
  • Immunoglobulin G (blood, immunology)
  • Immunoglobulin M (blood, immunology)
  • Male
  • Middle Aged
  • Mycobacterium tuberculosis (immunology, isolation & purification)
  • Polyproteins (immunology)
  • Tuberculosis (blood, diagnosis, drug therapy, immunology, microbiology)
  • Young Adult

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