We have shown in vitro that
thyroid hormones (THs) regulate the balance between proliferation and apoptosis of T
lymphoma cells. The effects of THs on
tumor development have been studied, but the results are still controversial. Herein, we show the modulatory action of thyroid status on the in vivo growth of T
lymphoma cells. For this purpose, euthyroid, hypothyroid, and
hyperthyroid mice received inoculations of EL4 cells to allow the development of solid
tumors.
Tumors in the
hyperthyroid animals exhibited a higher growth rate, as evidenced by the early appearance of palpable solid
tumors and the increased
tumor volume. These results are consistent with the rate of cell division determined by staining
tumor cells with
carboxyfluorescein succinimidyl
ester. Additionally,
hyperthyroid mice exhibited reduced survival. Hypothyroid mice did not differ significantly from the euthyroid controls with respect to these parameters. Additionally, only
tumors from
hyperthyroid animals had increased expression levels of
proliferating cell nuclear antigen and active
caspase 3. Differential expression of
cell cycle regulatory proteins was also observed. The levels of
cyclins D1 and D3 were augmented in the
tumors of the
hyperthyroid animals, whereas the cell cycle inhibitors
p16/INK4A (CDKN2A) and p27/Kip1 (CDKN1B) and the
tumor suppressor p53 (TRP53) were increased in hypothyroid mice. Intratumoral and peritumoral vasculogenesis was increased only in
hyperthyroid mice. Therefore, we propose that the thyroid status modulates the in vivo growth of EL4 T
lymphoma through the regulation of
cyclin,
cyclin-dependent kinase inhibitor, and tumor suppressor gene expression, as well as the stimulation of angiogenesis.