Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain.

Abstract

The contributing molecular pathways underlying the pathogenesis of breast cancer need to be better characterized. The principle of our study was to better understand the genetic mechanism of oncogenesis for human breast cancer and to discover new possible tumor markers for use in clinical practice. We used complimentary DNA (cDNA) microarrays to compare gene expression profiles of treated Michigan Cancer Foundation-7 (MCF-7) with recombinant bromelain and untreated MCF-7. SpringGene analysis was carried out of differential expression followed by Ingenuity Pathway Analysis (IPA), to understand the underlying consequence in developing disease and disorders. We identified 1,102 known genes differentially expressed to a significant degree (p<0.001) changed between the treatment. Within this gene set, 20 genes were significantly changed between treated cells and the control cells with cutoff fold change of more than 1.5. These genes are RNA-binding motif, single-stranded interacting protein 1 (RBMS1), ribosomal protein L29 (RPL29), glutathione S-transferase mu 2 (GSTM2), C15orf32, Akt3, B cell translocation gene 1 (BTG1), C6orf62, C7orf60, kinesin-associated protein 3 (KIFAP3), FBXO11, AT-rich interactive domain 4A (ARID4A), COPS2, TBPL1|SLC2A12, TMEM59, SNORD46, glioma tumor suppressor candidate region gene 2 (GLTSCR2), and LRRFIP. Our observation on gene expression indicated that recombinant bromelain produces a unique signature affecting different pathways, specific for each congener. The microarray results give a molecular mechanistic insight and functional effects, following recombinant bromelain treatment. The extent of changes in genes is related to and involved significantly in gap junction signaling, amyloid processing, cell cycle regulation by BTG family proteins, and breast cancer regulation by stathmin1 that play major roles.

• Authors: Nour Fouz, Azura Amid, Yumi Zuhanis Has-Yun Hashim
• Journal: Applied biochemistry and biotechnology (Appl Biochem Biotechnol) Vol. 173 Issue 7 Pg. 1618-39 (Aug 2014) ISSN: 1559-0291 [Electronic] United States
• PMID: 24928548 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Recombinant Proteins
• Bromelains
• stem bromelain

Topics

• Antineoplastic Agents (pharmacology)
• Breast Neoplasms (pathology)
• Bromelains (pharmacology)
• Cell Proliferation (drug effects)
• Gene Expression Profiling
• Genes, Neoplasm (genetics)
• Humans
• MCF-7 Cells
• Recombinant Proteins (pharmacology)