Radix Hedysari polysaccharide suppresses lipid metabolism dysfunction in a rat model of non‑alcoholic fatty liver disease via adenosine monophosphate‑activated protein kinase pathway activation.

Abstract

Oxidative stress and excess hepatic lipid accumulation contribute to non‑alcoholic fatty liver disease. Radix Hedysari polysaccharides (RHP) have attracted interest due to their antioxidant properties and immunomodulatory effects. However, the effect of RHP on hepatic lipid metabolism remains to be elucidated. In the present study, the response of Sprague‑Dawley rat livers to a high‑fat diet and RHP treatment was investigated by evaluating body weight, liver histology, hepatic lipid content, adenosine monophosphate‑activated protein kinase (AMPK) activity and lipid metabolism gene transcriptional profiles. The present study demonstrated that RHP ameliorated lipid metabolism disorders, regulated hepatic lipid content, improved liver inflammation and damage, activated AMPK via phosphorylation, upregulated peroxisome proliferator‑activated receptor α and downregulated the mRNA expression of sterol regulatory element binding protein‑1c in rat livers, which reduced lipogenesis and increased lipolysis. Taken together, these results suggested that RHP effectively ameliorates lipid metabolism disorders in rat livers; thus, RHP may be a potential therapeutic agent in the prevention of hepatic steatosis.

• Authors: Wei-Ming Sun, Yu-Ping Wang, Yong-Qiang Duan, Hong-Xia Shang, Wei-Dong Cheng
• Journal: Molecular medicine reports (Mol Med Rep) Vol. 10 Issue 3 Pg. 1237-44 (Sep 2014) ISSN: 1791-3004 [Electronic] Greece
• PMID: 24927063 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antioxidants
• Hypolipidemic Agents
• PPAR alpha
• Plant Extracts
• Polysaccharides
• RNA, Messenger
• Sterol Regulatory Element Binding Protein 1
• Transcription Factors
• AMP-Activated Protein Kinases

Topics

• AMP-Activated Protein Kinases (genetics, metabolism)
• Aconitum (chemistry)
• Animals
• Antioxidants (pharmacology)
• Body Weight (drug effects)
• Diet, High-Fat (adverse effects)
• Disease Models, Animal
• Down-Regulation
• Hypolipidemic Agents (pharmacology)
• Lipogenesis (drug effects)
• Liver (drug effects, metabolism)
• Male
• Non-alcoholic Fatty Liver Disease (drug therapy)
• Oxidative Stress (drug effects)
• PPAR alpha (genetics, metabolism)
• Plant Extracts (pharmacology)
• Polysaccharides (pharmacology)
• RNA, Messenger (genetics, metabolism)
• Rats
• Rats, Sprague-Dawley
• Signal Transduction
• Sterol Regulatory Element Binding Protein 1 (genetics, metabolism)
• Transcription Factors (genetics, metabolism)