NAT2, XRCC1 and hOGG1 polymorphisms, cigarette smoking, alcohol consumption and risk of upper aerodigestive tract cancer.

Abstract

AIM:

To evaluate associations between polymorphisms of the N-acetyltransferase 2 (NAT2), human 8-oxoguanine glycosylase 1 (hOGG1) and X-ray repair cross-complementing protein 1 (XRCC1) genes and risk of upper aerodigestive tract (UADT) cancer.

PATIENTS AND METHODS:

A case-control study involving 117 cases and 224 controls was undertaken. The NAT2 gene polymorphisms were genotyped by automated sequencing and XRCC1 Arg399Gln and hOGG1 Ser326Cys polymorphisms were determined by Polymerase Chain Reaction followed by Restriction Fragment Length Polymorphism (PCR-RFLP) methods.

RESULTS:

Slow metabolization phenotype was significantly associated as a risk factor for the development of UADT cancer (p=0.038). Furthermore, haplotype of slow metabolization was also associated with UADT cancer (p=0.014). The hOGG1 Ser326Cys polymorphism (CG or GG vs. CC genotypes) was shown as a protective factor against UADT cancer in moderate smokers (p=0.031). The XRCC1 Arg399Gln polymorphism (GA or AA vs. GG genotypes), in turn, was a protective factor against UADT cancer only among never-drinkers (p=0.048).

CONCLUSION:

Interactions involving NAT2, XRCC1 Arg399Gln and hOGG1 Ser326Cys polymorphisms may modulate the risk of UADT cancer in this population.

• Authors: Cintia Rodrigues Marques, Thiago Magalhães Da Silva, Dulcineia Martins De Albuquerque, Meiryane Santos Chaves, Marcilio Ferreira Marques Filho, Jamille Silva Oliveira, Giuliano Di Pietro, Sandra Mara Bispo Sousa, Aguinaldo Luiz Simões, Fabrício Rios-Santos
• Journal: Anticancer research (Anticancer Res) Vol. 34 Issue 6 Pg. 3217-24 (Jun 2014) ISSN: 1791-7530 [Electronic] Greece
• PMID: 24922697 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright© 2014 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.

Chemical References

• DNA-Binding Proteins
• X-ray Repair Cross Complementing Protein 1
• XRCC1 protein, human
• DNA
• Arylamine N-Acetyltransferase
• NAT2 protein, human
• DNA Glycosylases
• oxoguanine glycosylase 1, human

Topics

• Aged
• Alcohol Drinking (epidemiology)
• Arylamine N-Acetyltransferase (genetics)
• Case-Control Studies
• DNA (analysis)
• DNA Glycosylases (genetics)
• DNA-Binding Proteins (genetics)
• Female
• Follow-Up Studies
• Gastrointestinal Neoplasms (epidemiology)
• Humans
• Male
• Middle Aged
• Neoplasm Staging
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length
• Polymorphism, Single Nucleotide
• Prognosis
• Respiratory Tract Neoplasms (epidemiology)
• Risk Factors
• Smoking (epidemiology)
• X-ray Repair Cross Complementing Protein 1