Abstract | BACKGROUND: Previous studies showed that soluble LHCGR/hCG-sLHCGR concentrations in serum or plasma combined with PAPP-A and free βhCG significantly increased the sensitivity of Down's syndrome screen at early pregnancy without altering the false positive rate. The goal of the present study was to further examine the role of sLHCGR forms as combinatorial markers and to investigate whether sLHCGR could serve as an independent biomarker for Down's syndrome in first trimester pregnancy screens. METHODS: The PAPP-A, free βhCG, and hCG-sLHCGR concentrations together with nuchal translucency (NT) were measured in 40 Down's and 300 control pregnancies. The sLHCGR concentration was analysed in 40 Down's and 206 control pregnancies. RESULTS: The hCG-LHCGR in combination with PAPP-A and free βhCG increased the detection rate (DR) by 35% without altering the false positive rate (FPR). The sLHCGR: hCG-sLHCGR ratio alone detected 80% of Down's pregnancies in first trimester screening, with a false positive rate of 0.5%. CONCLUSIONS: While measurement of sLHCGR forms in combination with PAPP-A and free βhCG significantly increases the detection rate of Down's syndrome at first trimester, the ratio of sLHCGR: hCG-sLHCGR acts as an independent marker with a detection rate that is significantly higher than the existing biochemical markers individually for prenatal first trimester screening of Down's syndrome.
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Authors | Anne E Chambers, Walter E Mills, Imma Mercadé, Francesca Crovetto, Fatima Crispi, Laia Rodriguez-Revenga Bodi, Michael Pugia, Aurea Mira, Luis Lasalvia, Subhasis Banerjee, Elena Casals, Eduard Gratacos |
Journal | BMC pregnancy and childbirth
(BMC Pregnancy Childbirth)
Vol. 14
Pg. 197
(Jun 06 2014)
ISSN: 1471-2393 [Electronic] England |
PMID | 24906955
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers
- Chorionic Gonadotropin, beta Subunit, Human
- Receptors, LH
- Pregnancy-Associated Plasma Protein-A
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Topics |
- Area Under Curve
- Biomarkers
(blood)
- Case-Control Studies
- Chorionic Gonadotropin, beta Subunit, Human
(blood)
- Down Syndrome
(diagnosis)
- False Positive Reactions
- Female
- Humans
- Nuchal Translucency Measurement
- Predictive Value of Tests
- Pregnancy
- Pregnancy Trimester, First
(blood)
- Pregnancy-Associated Plasma Protein-A
(metabolism)
- Prenatal Diagnosis
(methods)
- ROC Curve
- Receptors, LH
(blood)
- Retrospective Studies
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