Abstract | BACKGROUND: METHODS: The effect of selected inhibitors on epigenetic modifications and viability of glioma C6 cells were studied using immunofluorescence and MTT metabolism test. RESULTS: We found that VPA and TSA increase histone H4 acetylation in glioma cells, while chaetocin and BIX01294 at low concentrations reduce H3K9me3, and 3DZNep decreases H3K27me3. Long-term treatment with some epigenetic inhibitors affects viability of glioma cells. CONCLUSIONS: We established the concentrations of selected inhibitors which in C6 glioma cells inhibit the enzyme activity, but do not decrease cell viability, hence allow to study the role of histone modifications in C6 glioma biology.
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Authors | Marta Maleszewska, Aleksandra Steranka, Bozena Kaminska |
Journal | Pharmacological reports : PR
(Pharmacol Rep)
Vol. 66
Issue 1
Pg. 107-13
(Feb 2014)
ISSN: 2299-5684 [Electronic] Switzerland |
PMID | 24905315
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved. |
Chemical References |
- Azepines
- BIX 01294
- Enzyme Inhibitors
- Histones
- Hydroxamic Acids
- Piperazines
- Quinazolines
- chaetocin
- trichostatin A
- 3-deazaneplanocin
- Valproic Acid
- Adenosine
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Topics |
- Acetylation
- Adenosine
(analogs & derivatives, pharmacology)
- Animals
- Azepines
(pharmacology)
- Brain Neoplasms
(enzymology, pathology)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Enzyme Inhibitors
(pharmacology)
- Epigenesis, Genetic
- Glioma
(enzymology, pathology)
- Histones
(metabolism)
- Hydroxamic Acids
(pharmacology)
- Piperazines
(pharmacology)
- Quinazolines
(pharmacology)
- Rats
- Valproic Acid
(pharmacology)
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