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MicroRNA-125a-3p expression in abdominal adipose tissues is associated with insulin signalling gene expressions in morbid obesity: observations in Taiwanese.

AbstractBACKGROUND:
Micro (mi) RNAs have been found to play an important role in the regulation of adipogenesis and insulin sensitivity. However, associations between miRNA and insulin signalling-related gene expressions in abdominal adipose tissues in obese subjects remain unclear.
METHODS:
We used a microarray platform to screen miRNA expressions in abdominal adipose tissues between genders in severely obese subjects and found that the top-ranking miRNA in abdominal omental adipose tissues was miRNA-125a-3p. MicroR-125a-3p and insulin signalling-related gene expressions in abdominal omental adipose tissues of all subjects (11 men and 10 women) were subsequently quantified by a real-time PCR. Also, associations of miR-125a-3p with insulin signalling-related gene expression and biochemical markers in obese subjects were analyzed by a linear regression analysis.
RESULTS:
miR-125a-3p expressed by abdominal omental adipose tissues was much higher in obese men than women. No gender difference was observed in abdominal subcutaneous adipose tissues. Concomitant with high miR-125a-3p, c-Jun N-terminal kinase gene expression was also higher, whereas insulin receptor was lower in men than women. There were negative associations of miR-125a-3p with the insulin receptor and phosphatidylinositol 3-kinase expressions. Fasting plasma glucose and cholesterol levels were positively associated with miR- 125a-3p expression. These associations were obvious in obese men but not women.
CONCLUSION:
Our results support the involvement of miR-125a-3p in regulating the insulin signalling pathway and imply that increased miR- 125a-3p expression in omental adipose tissues may be a characteristic feature of insulin resistance in obese men.
AuthorsChiu-Li Yeh, I-Chi Cheng, Yu-Chen Hou, Weu Wang, Sung-Ling Yeh
JournalAsia Pacific journal of clinical nutrition (Asia Pac J Clin Nutr) Vol. 23 Issue 2 Pg. 331-7 ( 2014) ISSN: 0964-7058 [Print] Australia
PMID24901105 (Publication Type: Journal Article)
Chemical References
  • Blood Glucose
  • Insulin
  • MIRN125 microRNA, human
  • MicroRNAs
  • Cholesterol
Topics
  • Abdominal Fat (metabolism)
  • Adult
  • Blood Glucose
  • Cholesterol
  • Female
  • Gene Expression (genetics)
  • Humans
  • Insulin (genetics, metabolism)
  • Male
  • MicroRNAs (genetics, metabolism)
  • Microarray Analysis (methods)
  • Middle Aged
  • Obesity, Morbid (genetics, metabolism)
  • Polymerase Chain Reaction (methods)
  • Sex Factors
  • Signal Transduction (genetics)
  • Taiwan
  • Young Adult

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