Abstract | BACKGROUND: Micro (mi) RNAs have been found to play an important role in the regulation of adipogenesis and insulin sensitivity. However, associations between miRNA and insulin signalling-related gene expressions in abdominal adipose tissues in obese subjects remain unclear. METHODS: We used a microarray platform to screen miRNA expressions in abdominal adipose tissues between genders in severely obese subjects and found that the top-ranking miRNA in abdominal omental adipose tissues was miRNA-125a-3p. MicroR-125a-3p and insulin signalling-related gene expressions in abdominal omental adipose tissues of all subjects (11 men and 10 women) were subsequently quantified by a real-time PCR. Also, associations of miR-125a-3p with insulin signalling-related gene expression and biochemical markers in obese subjects were analyzed by a linear regression analysis. RESULTS: miR-125a-3p expressed by abdominal omental adipose tissues was much higher in obese men than women. No gender difference was observed in abdominal subcutaneous adipose tissues. Concomitant with high miR-125a-3p, c-Jun N-terminal kinase gene expression was also higher, whereas insulin receptor was lower in men than women. There were negative associations of miR-125a-3p with the insulin receptor and phosphatidylinositol 3-kinase expressions. Fasting plasma glucose and cholesterol levels were positively associated with miR- 125a-3p expression. These associations were obvious in obese men but not women. CONCLUSION: Our results support the involvement of miR-125a-3p in regulating the insulin signalling pathway and imply that increased miR- 125a-3p expression in omental adipose tissues may be a characteristic feature of insulin resistance in obese men.
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Authors | Chiu-Li Yeh, I-Chi Cheng, Yu-Chen Hou, Weu Wang, Sung-Ling Yeh |
Journal | Asia Pacific journal of clinical nutrition
(Asia Pac J Clin Nutr)
Vol. 23
Issue 2
Pg. 331-7
( 2014)
ISSN: 0964-7058 [Print] Australia |
PMID | 24901105
(Publication Type: Journal Article)
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Chemical References |
- Blood Glucose
- Insulin
- MIRN125 microRNA, human
- MicroRNAs
- Cholesterol
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Topics |
- Abdominal Fat
(metabolism)
- Adult
- Blood Glucose
- Cholesterol
- Female
- Gene Expression
(genetics)
- Humans
- Insulin
(genetics, metabolism)
- Male
- MicroRNAs
(genetics, metabolism)
- Microarray Analysis
(methods)
- Middle Aged
- Obesity, Morbid
(genetics, metabolism)
- Polymerase Chain Reaction
(methods)
- Sex Factors
- Signal Transduction
(genetics)
- Taiwan
- Young Adult
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