Metabolic engineering of Escherichia coli to enhance shikimic acid production from sorbitol.

Abstract

Shikimic acid (SA) is the key synthetic material of Oseltamivir, which is an effective drug for the prevention and treatment of influenza. In this study, to block the downstream metabolic pathway of SA, the shikimate kinase isoenzyme genes aroK and aroL were deleted by Red recombination. Moreover, the key enzyme genes aroG, aroB, tktA and aroE of SA pathway were co-expressed by constructing the recombinant vector pETDuet-GBAE. As a result, SA production of E. coli BW25113 (∆aroL/aroK, DE3)/pETDuet-GBAE reached 1,077.6 mg/l when low amounts of sorbitol (5 g/l) were fed in shake flasks. The yield was 3.7 times that when glucose was used (P < 0.05). The results showed that sorbitol was an optimized carbon source for the high efficient accumulation of SA for the first time, which was applicable to use in the industry for high yields and low consumption.

• Authors: Xianglei Liu, Jun Lin, Haifeng Hu, Bin Zhou, Baoquan Zhu
• Journal: World journal of microbiology & biotechnology (World J Microbiol Biotechnol) Vol. 30 Issue 9 Pg. 2543-50 (Sep 2014) ISSN: 1573-0972 [Electronic] Germany
• PMID: 24894540 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Recombinant Proteins
• Shikimic Acid
• Sorbitol
• Carbon

Topics

• Carbon (metabolism)
• Escherichia coli (genetics, metabolism)
• Gene Deletion
• Gene Expression
• Metabolic Engineering
• Metabolic Networks and Pathways (genetics)
• Plasmids
• Recombinant Proteins (genetics, metabolism)
• Shikimic Acid (metabolism)
• Sorbitol (metabolism)