Using infective mosquitoes to challenge monkeys with Plasmodium knowlesi in malaria vaccine studies.
Abstract | BACKGROUND: METHODS: Several species and strains of mosquitoes were tested for their ability to produce Plasmodium knowlesi sporozoites. Donor monkey parasitaemia effects on oocyst and sporozoite numbers and mosquito mortality were documented. Methylparaben added to mosquito feed was tested to improve mosquito survival. To determine the number of bites needed to infect a monkey, animals were exposed to various numbers of P. knowlesi-infected mosquitoes. Finally, P. knowlesi-infected mosquitoes were used to challenge 17 monkeys in a malaria vaccine trial, and the effect of number of infectious bites on monkey parasitaemia was documented. RESULTS: Anopheles dirus, Anopheles crascens, and Anopheles dirus X (a cross between the two species) produced large numbers of P. knowlesi sporozoites. Mosquito survival to day 14, when sporozoites fill the salivary glands, averaged only 32% when donor monkeys had a parasitaemia above 2%. However, when donor monkey parasitaemia was below 2%, mosquitoes survived twice as well and contained ample sporozoites in their salivary glands. Adding methylparaben to sugar solutions did not improve survival of infected mosquitoes. Plasmodium knowlesi was very infectious, with all monkeys developing blood stage infections if one or more infected mosquitoes successfully fed. There was also a dose-response, with monkeys that received higher numbers of infected mosquito bites developing malaria sooner. CONCLUSIONS: Anopheles dirus, An. crascens and a cross between these two species all were excellent vectors for P. knowlesi. High donor monkey parasitaemia was associated with poor mosquito survival. A single infected mosquito bite is likely sufficient to infect a monkey with P. knowlesi. It is possible to efficiently challenge large groups of monkeys by mosquito bite, which will be useful for P. knowlesi vaccine studies.
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Authors | Jittawadee R Murphy, Walter R Weiss, David Fryauff, Megan Dowler, Tatyana Savransky, Cristina Stoyanov, Olga Muratova, Lynn Lambert, Sachy Orr-Gonzalez, Katie Lynn Zeleski, Jessica Hinderer, Michael P Fay, Gyan Joshi, Robert W Gwadz, Thomas L Richie, Eileen Franke Villasante, Jason H Richardson, Patrick E Duffy, Jingyang Chen |
Journal | Malaria journal
(Malar J)
Vol. 13
Pg. 215
(Jun 03 2014)
ISSN: 1475-2875 [Electronic] England |
PMID | 24893777
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, N.I.H., Intramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
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Topics |
- Animals
- Anopheles
(parasitology, physiology)
- Female
- Macaca mulatta
- Malaria
(transmission)
- Malaria Vaccines
(administration & dosage)
- Male
- Plasmodium knowlesi
(growth & development)
- Survival Analysis
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