Inhibition of tumour cell adhesion by anti-metastatic polypeptide containing a repetitive Arg-Gly-Asp sequence.

Abstract

Anti-cell adhesive activity was examined by the synthetic polypeptide, containing repetitive Arg-Gly-Asp sequence of cell attachment site from fibronectin, poly (Arg-Gly-Asp). The attachment of tumour cells to fibronectin substrate was specifically inhibited by adding poly (Arg-Gly-Asp) in cell surface receptor-mediated and divalent cation-dependent manners, but not by unrelated peptides. In our previous study, the lung metastatic formation of tumour cells was dramatically reduced by intravenous co-injection of anti-cell adhesive poly (Arg-Gly-Asp) with B16-BL6 melanoma cells. These findings suggest that polypeptide-mediated inhibition of pulmonary metastasis is partly due to interference with tumour cell adhesion to the substrates including fibronectin in target organs or tissues.

• Authors: J Murata, I Saiki, J Iida, I Azuma, H Kawahara, N Nishi, S Tokura
• Journal: International journal of biological macromolecules (Int J Biol Macromol) Vol. 11 Issue 4 Pg. 226-32 (Aug 1989) ISSN: 0141-8130 [Print] Netherlands
• PMID: 2489085 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fibronectins
• Oligopeptides
• Peptides
• arginyl-glycyl-aspartic acid

Topics

• Amino Acid Sequence
• Animals
• Cell Adhesion (drug effects)
• Female
• Fibronectins (metabolism)
• Melanoma, Experimental (metabolism, secondary)
• Mice
• Mice, Inbred C57BL
• Molecular Sequence Data
• Neoplasm Metastasis
• Oligopeptides (metabolism, pharmacology)
• Peptides (chemistry, metabolism, pharmacology)
• Repetitive Sequences, Nucleic Acid
• Substrate Specificity
• Tumor Cells, Cultured