Zerumbone suppresses IL-1β-induced cell migration and invasion by inhibiting IL-8 and MMP-3 expression in human triple-negative breast cancer cells.

Abstract

Inflammation is a key regulatory process in cancer development. Prolonged exposure of breast tumor cells to inflammatory cytokines leads to epithelial-mesenchymal transition, which is the principal mechanism involved in metastasis and tumor invasion. Interleukin (IL)-1β is a major inflammatory cytokine in a variety of tumors. To date, the regulatory mechanism of IL-1β-induced cell migration and invasion has not been fully elucidated. Here, we investigated the effect of zerumbone (ZER) on IL-1β-induced cell migration and invasion in breast cancer cells. The levels of IL-8 and matrix metalloproteinase (MMP)-3 mRNA were analyzed by real-time polymerase chain reaction. The levels of secreted IL-8 and MMP-3 protein were analyzed by enzyme-linked immunosorbent assay and western blot analysis, respectively. Cell invasion and migration was detected by Boyden chamber assay. The levels of IL-8 and MMP-3 expression were significantly increased by IL-1β treatment in Hs578T and MDA-MB231 cells. On the other hand, IL-1β-induced IL-8 and MMP-3 expression was decreased by ZER. Finally, IL-1β-induced cell migration and invasion were decreased by ZER in Hs578T and MDA-MB231 cells. ZER suppresses IL-1β-induced cell migration and invasion by inhibiting IL-8 expression and MMP-3 expression in TNBC cells. ZER could be a promising therapeutic drug for treatment of triple-negative breast cancer patients.

• Authors: Jeonghun Han, Soo Youn Bae, Soo-Jin Oh, Jeongmin Lee, Jun Ho Lee, Hyun-Chul Lee, Se Kyung Lee, Won Ho Kil, Seok Won Kim, Seok Jin Nam, Sangmin Kim, Jeong Eon Lee
• Journal: Phytotherapy research : PTR (Phytother Res) Vol. 28 Issue 11 Pg. 1654-60 (Nov 2014) ISSN: 1099-1573 [Electronic] England
• PMID: 24890258 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 John Wiley & Sons, Ltd.

Chemical References

• CXCL8 protein, human
• Interleukin-1beta
• Interleukin-8
• Sesquiterpenes
• zerumbone
• MMP3 protein, human
• Matrix Metalloproteinase 3

Topics

• Cell Line, Tumor
• Cell Movement (drug effects)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Interleukin-1beta (pharmacology)
• Interleukin-8 (metabolism)
• Matrix Metalloproteinase 3 (metabolism)
• Neoplasm Invasiveness
• Sesquiterpenes (pharmacology)
• Triple Negative Breast Neoplasms (metabolism, pathology)