Effects of diallyl trisulfide on induction of apoptotic death in murine leukemia WEHI-3 cells in vitro and alterations of the immune responses in normal and leukemic mice in vivo.

Abstract

Diallyl trisulfide (DATS), a chemopreventive dietary constituent and extracted from garlic, has been shown to against cultured many types of human cancer cell liens but the fate of apoptosis in murine leukemia cells in vitro and immune responses in leukemic mice remain elusive. Herein, we clarified the actions of DATS on growth inhibition of murine leukemia WEHI-3 cells in vitro and used WEHI-3 cells to generate leukemic mice in vivo, following to investigate the effects of DATS in animal model. In in vitro study, DATS induced apoptosis of WEHI-3 cells through the G0/G1 phase arrest and induction of caspase-3 activation. In in vivo study DATS decreased the weight of spleen of leukemia mice but did not affect the spleen weight of normal mice. DATS promoted the immune responses such as promotions of the macrophage phagocytosis and NK cell activities in WEHI-3 leukemic and normal mice. However, DATS only promotes NK cell activities in normal mice. DATS increases the surface markers of CD11b and Mac-3 in leukemia mice but only promoted CD3 in normal mice. In conclusion, the present study indicates that DATS induces cell death through induction of apoptosis in mice leukemia WHEI-3 cells. DATS also promotes immune responses in leukemia and normal mice in vivo.

• Authors: Fang-Ming Hung, Hung-Sheng Shang, Nou-Ying Tang, Jen-Jyh Lin, Kung-Wen Lu, Jing-Pin Lin, Yang-Ching Ko, Chien-Chih Yu, Hai-Lung Wang, Jung-Chi Liao, Hsu-Feng Lu, Jing-Gung Chung
• Journal: Environmental toxicology (Environ Toxicol) Vol. 30 Issue 11 Pg. 1343-53 (Nov 2015) ISSN: 1522-7278 [Electronic] United States
• PMID: 24890016 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 Wiley Periodicals, Inc.

Chemical References

• Allyl Compounds
• Anticarcinogenic Agents
• Antigens, Differentiation
• Sulfides
• monocyte-macrophage differentiation antigen
• diallyl trisulfide
• Caspase 3

Topics

• Allyl Compounds (pharmacology, therapeutic use)
• Animals
• Anticarcinogenic Agents (pharmacology, therapeutic use)
• Antigens, Differentiation (immunology)
• Apoptosis (drug effects)
• Caspase 3 (metabolism)
• Cell Cycle Checkpoints (drug effects)
• Cell Line, Tumor
• Cell Survival (drug effects)
• Cytotoxicity, Immunologic (drug effects)
• Garlic (chemistry)
• Killer Cells, Natural (drug effects, immunology)
• Leukemia, Experimental (immunology, prevention & control)
• Lymphocyte Activation (drug effects)
• Macrophages, Peritoneal (drug effects, immunology)
• Mice
• Mice, Inbred BALB C
• Neoplasm Transplantation
• Phagocytosis (drug effects, immunology)
• Spleen (drug effects, immunology)
• Sulfides (pharmacology, therapeutic use)