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Development of an immunochromatography strip test based on truncated nucleocapsid antigens of three representative hantaviruses.

AbstractBACKGROUND:
Hantaviruses are causative agents of hemorrhagic fever with renal syndrome (HFRS) and nephropathia epidemica (NE) in the Old World and hantavirus pulmonary syndrome (HPS) in the New World. There is a need for time-saving diagnostic methods. In the present study, recombinant N antigens were used as antigens in an immunochromatography strip (ICG) test to detect specific IgG antibodies.
METHODS:
The N-terminal 103 amino acids (aa) of Hantaan virus (HTNV), Puumala virus (PUUV) and Andes virus (ANDV) nucleocapsid (N) protein were expressed in E. coli as representative antigens of three groups (HFRS, NE and HPS-causing viruses) of hantavirus. Five different types of ICG test strips, one antigen line on one strip for each of the three selected hantaviruses (HTNV, PUUV and ANDV), three antigen lines on one strip and a mixed antigen line on one strip, were developed and sensitivities were compared.
RESULTS:
A total of 87 convalescent-phase patient sera, including sera from 35 HFRS patients, 36 NE patients and 16 HPS patients, and 25 sera from healthy seronegative people as negative controls were used to evaluate the ICG test. Sensitivities of the three-line strip and mixed-line strip were similar to those of the single antigen strip (97.2 to 100%). On the other hand, all of the ICG test strips showed high specificities to healthy donors.
CONCLUSION:
These results indicated that the ICG test with the three representative antigens is an effective serodiagnostic tool for screening and typing of hantavirus infection in humans.
AuthorsTakako Amada, Kumiko Yoshimatsu, Takaaki Koma, Kenta Shimizu, Chandika D Gamage, Kanae Shiokawa, Sanae Nishio, Clas Ahlm, Jiro Arikawa
JournalVirology journal (Virol J) Vol. 11 Pg. 87 ( 2014) ISSN: 1743-422X [Electronic] England
PMID24885901 (Publication Type: Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Viral
  • Antigens, Viral
  • Immunoglobulin G
  • Nucleocapsid Proteins
  • Recombinant Proteins
Topics
  • Antibodies, Viral (blood)
  • Antigens, Viral (genetics, isolation & purification)
  • Escherichia coli (genetics)
  • Gene Expression
  • Hantaan virus (genetics, immunology)
  • Hantavirus (genetics, immunology)
  • Hantavirus Infections (diagnosis, virology)
  • Humans
  • Immunochromatography (methods)
  • Immunoglobulin G (blood)
  • Nucleocapsid Proteins (genetics, isolation & purification)
  • Puumala virus (genetics, immunology)
  • Recombinant Proteins (genetics, isolation & purification)
  • Sensitivity and Specificity

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