Abstract | BACKGROUND: METHODS: We studied hnRNP K-regulated MMPs by analyzing the expression profiles of MMP family genes in NPC tissues and hnRNP K-knockdown NPC cells using Affymetrix microarray analysis and quantitative RT-PCR. The association of hnRNP K and MMP12 expression in 82 clinically proven NPC cases was determined by immunohistochemical analysis. The hnRNP K-mediated MMP12 regulation was determined by zymography and Western blot, as well as by promoter, DNA pull-down and chromatin immunoprecipitation (ChIP) assays. The functional role of MMP12 in cell migration and invasion was demonstrated by MMP12-knockdown and the treatment of MMP12-specific inhibitor, PF-356231. RESULTS: MMP12 was overexpressed in NPC tissues, and this high level of expression was significantly correlated with high-level expression of hnRNP K (P = 0.026). The levels of mRNA, protein and enzyme activity of MMP12 were reduced in hnRNP K-knockdown NPC cells. HnRNP K interacting with the region spanning -42 to -33 bp of the transcription start site triggered transcriptional activation of the MMP12 promoter. Furthermore, inhibiting MMP12 by MMP12 knockdown and MMP12-specific inhibitor, PF-356231, significantly reduced the migration and invasion of NPC cells. CONCLUSIONS: Overexpression of MMP12 was significantly correlated with hnRNP K in NPC tissues. HnRNP K can induce MMP12 expression and enzyme activity through activating MMP12 promoter, which promotes cell migration and invasion in NPC cells. In vitro experiments suggest that NPC metastasis with high MMP12 expression may be treated with PF-356231. HnRNP K and MMP12 may be potential therapeutic markers for NPC, but additional validation studies are warranted.
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Authors | I-Che Chung, Lih-Chyang Chen, An-Ko Chung, Mei Chao, Hsin-Yi Huang, Chuen Hsueh, Ngan-Ming Tsang, Kai-Ping Chang, Ying Liang, Hsin-Pai Li, Yu-Sun Chang |
Journal | BMC cancer
(BMC Cancer)
Vol. 14
Pg. 348
(May 20 2014)
ISSN: 1471-2407 [Electronic] England |
PMID | 24885469
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Heterogeneous-Nuclear Ribonucleoprotein K
- Matrix Metalloproteinase Inhibitors
- RNA, Messenger
- Ribonucleoproteins
- HNRNPK protein, human
- MMP12 protein, human
- Matrix Metalloproteinase 12
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Topics |
- Adult
- Aged
- Carcinoma
- Cell Line, Tumor
- Cell Movement
(drug effects)
- Enzyme Induction
- Female
- Heterogeneous-Nuclear Ribonucleoprotein K
- Humans
- Male
- Matrix Metalloproteinase 12
(biosynthesis, genetics)
- Matrix Metalloproteinase Inhibitors
(pharmacology)
- Middle Aged
- Nasopharyngeal Carcinoma
- Nasopharyngeal Neoplasms
(enzymology, genetics, pathology)
- Neoplasm Invasiveness
- Promoter Regions, Genetic
- RNA Interference
- RNA, Messenger
(biosynthesis)
- Retrospective Studies
- Ribonucleoproteins
(genetics, metabolism)
- Signal Transduction
- Time Factors
- Transcriptional Activation
- Transfection
- Young Adult
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