Kaliziri extract upregulates tyrosinase, TRP-1, TRP-2 and MITF expression in murine B16 melanoma cells.

Abstract

BACKGROUND:

Kaliziri extract (KZE) is a traditional Uyghur medicine (TUM), used by traditional hospitals in China as an injection for treatment of vitiligo for more than 30 years. Clinical application has shown that this medicine has obvious therapeutic effects. However, its phytochemical analysis and mechanism have not been examined.

METHODS:

KZE was extracted from seeds of Kaliziri [Vernonia anthelmintica (L.) Willd.] in ethanol-water (80:20, v/v), its components were identified by LC-MS/MS, and the signaling pathway of melanin synthesis in KZE treated murine B16 melanoma cells was examined by western blotting.

RESULTS:

Liquid chromatography-mass spectrometry analysis confirmed that the main components of KZE are flavonoids. KZE increased the tyrosinase activity and melanin content in a dose-dependent manner at concentrations of 5-40 μg/ml, and treatment with 20 μg/ml of KZE enhanced the expression of tyrosinase in B16 cells in a time-dependent manner.

CONCLUSIONS:

KZE induced melanogenesis by increasing the expression of TYR, TRP-1, TRP-2 and MITF in B16 cells.

• Authors: Adila Tuerxuntayi, Yong-qiang Liu, Ablajan Tulake, Maidina Kabas, Aiden Eblimit, Haji Akber Aisa
• Journal: BMC complementary and alternative medicine (BMC Complement Altern Med) Vol. 14 Pg. 166 (May 22 2014) ISSN: 1472-6882 [Electronic] England
• PMID: 24884952 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Microphthalmia-Associated Transcription Factor
• Mitf protein, mouse
• Plant Extracts
• Oxidoreductases
• tyrosinase-related protein-1
• Monophenol Monooxygenase
• Intramolecular Oxidoreductases
• dopachrome isomerase

Topics

• Animals
• Cell Line, Tumor
• Cell Survival (drug effects)
• Intramolecular Oxidoreductases (biosynthesis, genetics)
• Melanoma, Experimental (enzymology, genetics, metabolism)
• Mice
• Microphthalmia-Associated Transcription Factor (biosynthesis, genetics)
• Monophenol Monooxygenase (biosynthesis, genetics)
• Oxidoreductases (biosynthesis, genetics)
• Plant Extracts (chemistry, pharmacology)
• Up-Regulation (drug effects)
• Vernonia (chemistry)