Abstract |
Ploidy was determined on 663 resectable primary tumors from untreated patients. Nuclei obtained by mechanical disaggregation of frozen tissue were stained with propidium iodide and analysed in a FACS IV. Aneuploidy was detected in 73% of cases. It was not significantly related to nodal involvement or tumor size, although the highest frequencies were observed in large tumors (88%) or with more than 10 positive nodes (77%). Aneuploidy was more frequently observed in ductal infiltrating (81%) than in lobular histology and in tumors lacking both progesterone and estrogen receptors (85%). Analysis of ploidy in primary and synchronous lymph node metastases from the same patient showed a high agreement rate (90%) of DNA patterns simply defined as diploid or aneuploid. However, differences in DNA stemlines and DNA indices between the two synchronous lesions from the same patient were a rather frequent event.
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Authors | G Del Bino, R Silvestrini, M R Zucconi, G Marchese, B Valentinis, G Di Fronzo, S Andreola |
Journal | Analytical cellular pathology : the journal of the European Society for Analytical Cellular Pathology
(Anal Cell Pathol)
Vol. 1
Issue 4
Pg. 215-23
(Aug 1989)
ISSN: 0921-8912 [Print] Netherlands |
PMID | 2487046
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- DNA, Neoplasm
- Receptors, Estrogen
- Receptors, Progesterone
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Breast Neoplasms
(chemistry, genetics, pathology)
- Cell Fractionation
(methods)
- DNA, Neoplasm
(analysis)
- Female
- Freezing
- Humans
- Lymphatic Metastasis
- Middle Aged
- Ploidies
- Receptors, Estrogen
(analysis)
- Receptors, Progesterone
(analysis)
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