Abstract |
Etimicin intermediate 3,2″,6″-N,N,N-triacetyl gentamicin C1a (P1), is a key intermediate of etimicin, which is a semi-synthetic aminoglycoside antibiotic effective to both gram-positive and gram-negative bacteria infections. Four major related impurities of P1 were detected by HPLC-ELSD and ESI-MS(n) methods. Weakly acidic cation exchange resin, CM- sephadex and silica gel column chromatography were used for the isolation and purification of four major impurities. By means of ESI-MS(n) and NMR analysis, related impurities were characterized as 3,2″-N,N-diacetyl gentamicin C1a (1), 3,2″,6″-N,N,N-triacetyl gentamicin C2b (2), 2″-N-acetyl gentamicin C1a (3), and 2″,6″-N,N-diacetyl gentamicin C1a (4). Impurities 1, 2, 4 are novel compounds and the NMR data of these isolates were first reported in this paper. The possible mechanism for the formation of these impurities is also discussed.
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Authors | Yuanchao Wang, Zhijie Zhang, Lingyun Wu, Xiaoqi Zhang, Hao Wang, Wencai Ye, Ping Li |
Journal | Journal of pharmaceutical and biomedical analysis
(J Pharm Biomed Anal)
Vol. 97
Pg. 97-102
(Aug 2014)
ISSN: 1873-264X [Electronic] England |
PMID | 24858300
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2014 Elsevier B.V. All rights reserved. |
Chemical References |
- Aminoglycosides
- Gentamicins
- etimicin
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Topics |
- Aminoglycosides
(chemical synthesis, chemistry)
- Chromatography, Liquid
- Drug Contamination
- Gentamicins
(chemistry, isolation & purification)
- Magnetic Resonance Spectroscopy
- Molecular Structure
- Spectrometry, Mass, Electrospray Ionization
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