Phosphorylcholine and SpaA, a choline-binding protein, are involved in the adherence of Erysipelothrix rhusiopathiae to porcine endothelial cells, but this adherence is not mediated by the PAF receptor.

Abstract

A crucial event in the initiation of many bacterial infections is the adherence of the bacteria to host cells, and bacterial surface structures and their interactions with host cell receptors play an important role in this process. Erysipelothrix rhusiopathiae is the causative agent of swine erysipelas, which may cause acute septicemia or chronic endocarditis and polyarthritis. To study the pathogenic mechanism of the widespread vascular disease observed in the acute form of swine erysipelas, we investigated the role of phosphorylcholine (PCho), a component of the E. rhusiopathiae capsule, in bacterial adherence to porcine endothelial cells (PECs) in vitro. We found that adherence of E. rhusiopathiae strain Fujisawa to PECs was twice that of adherence to control COS-7 cells and that the adherence rates of PCho-defective mutants were approximately 30-50% lower than those of the Fujisawa strain. The adherence of the Fujisawa strain to COS-7 cells transfected with the porcine platelet-activating factor receptor (PAFR) gene, which encodes a G protein-coupled receptor that has been shown to directly bind to Streptococcus pneumoniae via PCho in the bacterial cell wall, was not enhanced. Treatment with a PAFR antagonist (WEB-2086) did not inhibit bacterial adherence to PECs. Incubation of the bacterial cells with an antibody against PCho or SpaA, a choline-binding protein anchored to PCho of the Fujisawa strain, reduced the adherence of the strain to PECs. This effect was not observed when PCho-defective mutants were used. These results suggest that E. rhusiopathiae adheres to PECs via PCho and SpaA and that the PCho-mediated adherence is independent of PAFR.

• Authors: Tomoyuki Harada, Yohsuke Ogawa, Masahiro Eguchi, Fang Shi, Masumi Sato, Kazuyuki Uchida, Hiroyuki Nakayama, Yoshihiro Shimoji
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 172 Issue 1-2 Pg. 216-22 (Aug 06 2014) ISSN: 1873-2542 [Electronic] Netherlands
• PMID: 24856134 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2014 Elsevier B.V. All rights reserved.

Chemical References

• Antibodies
• Antigens, Bacterial
• Azepines
• Bacterial Proteins
• Platelet Membrane Glycoproteins
• Receptors, G-Protein-Coupled
• SpaA protein, Erysipelothrix rhusiopathiae
• Triazoles
• platelet activating factor receptor
• WEB 2086
• Phosphorylcholine

Topics

• Animals
• Antibodies (pharmacology)
• Antigens, Bacterial (genetics, metabolism)
• Azepines (pharmacology)
• Bacterial Adhesion (drug effects)
• Bacterial Capsules (metabolism)
• Bacterial Proteins (antagonists & inhibitors, genetics, metabolism)
• COS Cells
• Chlorocebus aethiops
• Endothelial Cells (drug effects, metabolism, microbiology)
• Erysipelothrix (genetics, metabolism)
• Gene Expression
• Host Specificity
• Phosphorylcholine (antagonists & inhibitors, metabolism)
• Platelet Membrane Glycoproteins (antagonists & inhibitors, genetics, metabolism)
• Protein Binding
• Receptors, G-Protein-Coupled (antagonists & inhibitors, genetics, metabolism)
• Swine
• Triazoles (pharmacology)