Additive interactions between 1-methyl-1,2,3,4-tetrahydroisoquinoline and clobazam in the mouse maximal electroshock-induced tonic seizure model--an isobolographic analysis for parallel dose-response relationship curves.

Abstract

BACKGROUND:

The aim of this study was to characterize the anticonvulsant effect of 1-methyl-1,2,3,4-tetrahydroisoquinoline (1-MeTHIQ) in combination with clobazam (CLB) in the mouse maximal electroshock-induced seizure (MES) model.

METHODS:

The anticonvulsant interaction profile between 1-MeTHIQ and CLB in the mouse MES model was determined using an isobolographic analysis for parallel dose-response relationship curves.

RESULTS:

Electroconvulsions were produced in albino Swiss mice by a current (sine wave, 25 mA, 500 V, 50 Hz, 0.2-second stimulus duration) delivered via auricular electrodes by a Hugo Sachs generator. There was an additive effect of the combination of 1-MeTHIQ with CLB (at the fixed ratios of 1:3, 1:1 and 3:1) in the mouse MES-induced tonic seizure model.

CONCLUSIONS:

The additive interaction of the combination of 1-MeTHIQ with CLB (at fixed-ratios of 1:3, 1:1 and 3:1) in the mouse MES model seems to be pharmacodynamic in nature and worth of considering in further clinical practice.

• Authors: Marta Andres-Mach, Agnieszka Haratym-Maj, Mirosław Zagaja, Jarogniew J Luszczki
• Journal: Pharmacology (Pharmacology) Vol. 93 Issue 3-4 Pg. 172-7 ( 2014) ISSN: 1423-0313 [Electronic] Switzerland
• PMID: 24853974 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2014 S. Karger AG, Basel.

Chemical References

• Anticonvulsants
• Tetrahydroisoquinolines
• Benzodiazepines
• Clobazam
• 1-methyl-1,2,3,4-tetrahydroisoquinoline

Topics

• Animals
• Anticonvulsants (administration & dosage, pharmacology)
• Benzodiazepines (administration & dosage, pharmacology)
• Clobazam
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Drug Interactions
• Drug Synergism
• Drug Therapy, Combination
• Electroshock
• Male
• Mice
• Seizures (drug therapy, physiopathology)
• Tetrahydroisoquinolines (administration & dosage, pharmacology)